HOG MAPK経路の一時的な活性化は,バイモダル遺伝子発現を調節する
Serge Pelet1, Fabian Rudolf, Mariona Nadal-Ribelles
1ETH-Zurich, Department of Biology, Institute of Biochemistry, Schafmattstrasse 18, CH-8093 Zurich, Switzerland. serge.pelet@bc.biol.ethz.ch
Science (New York, N.Y.)
|May 10, 2011
まとめ
線形信号は,バイモダルの遺伝子発現反応を誘発する. 転写の遅いストキャスティックスイッチは,一時的なp38/Hog1 MAPK活性化と組み合わせて,オスモティックストレス反応のための細胞値を作成します.
科学分野:
- 細胞の信号伝達経路は,
- 分子生物学は分子生物学である.
- システム生物学 システム生物学
背景:
- ミトゲン活性化タンパク質キナーゼ (MAPK) カスケードは,外部および内部信号を統合して,細胞プロセスに不可欠です.
- p38/Hog1 MAPK経路は,オスモティックストレスによって活性化され,核転位と特定の遺伝子発現の変化を引き起こします.
研究 の 目的:
- Hog1の活性化とその下流の遺伝子発現の間のダイナミックな関係を調査する.
- 線形シグナル伝達に対する応答として観測された転写出力の基礎となる分子機構を解明する.
主な方法:
- 信号ダイナミクスの数学モデリング.
- 細胞反応を観察するための単細胞実験.
- 暫定的なMAPK活性化と転写状態の相互作用の分析.
主要な成果:
- Hog1の活性化は,刺激の強度との線形相関を示している.
- トランスクリプションの出力は,Hog1の活性化に直接比例しないバイモダル反応を示します.
- 抑制された状態と活性化された転写状態の間のゆっくりとストキャスティックな移行が特定されました.
結論:
- バイモダル転写出力は,一時的なHog1活性化と遅いストキャスティック転写スイッチの組み合わせから生じる.
- このメカニズムは,細胞がトランスクリプションの値を確立し,線形信号入力に非線形で反応することを可能にします.
- 細胞が環境のシグナルに反応して遺伝子発現の正確な制御をどのように達成するかについての洞察を提供します.
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