哺乳類のTATA-box配列の機能的異質性は,細胞特異の強化剤との相互作用によって明らかになりました
F C Wefald1, B H Devlin, R S Williams
1Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Nature
|March 15, 1990
まとめ
筋肉特異増強剤 (MSE) は,遺伝子プロモーターと異なる相互作用をする. TATAボックス配列の差異は,MSEが遺伝子転写を活性化できるかどうかを決定し,TATAボックス要素の機能的異質性を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- 筋肉特異増強剤 (MSE) は,ヒトミオグロビン遺伝子を調節する.
- MSEはミオグロビン核プロモーターと機能するが,シミアンウイルス40 (SV40) の早期プロモーターは機能しない.
- プロモーターの違いは,そのTATAボックス配列にある:TATAAAA (ミオグルビン) vs.TATTTAT (SV40).
研究 の 目的:
- TATAボックス配列の変異が,MSEに対するディフェンシャルプロモーター応答を引き起こすかどうかを調査する.
- MSE媒介の遺伝子調節におけるTATAボックス配列の役割を決定する.
主な方法:
- サイト指向型変異は,ミオグルビンとSV40プロモーターのTATAボックス配列を変更するために使用されました.
- MSEの存在下でのプロモーター活性を評価するために,レポーター遺伝子アッセイが行われました.
主要な成果:
- ミオグロビンTATAボックスをSV40配列に変更することで,MSE反応性は廃止されました.
- SV40 TATA ボックスをミオグロビン配列に切り替えると,MSE反応性が認められた.
- これらの発見は,TATAボックス配列がMSEの相互作用にとって重要であることを示しています.
結論:
- 哺乳類のTATA-box要素は,機能的な異質性を示しています.
- この異質性は,TATAボックス結合因子の異なる結合から生じる可能性が高い.
- 特定のTATAボックス結合因子は,MSE結合タンパク質と協働して転写活性化を起こす可能性があります.
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