人間のT細胞受容体デルタロカス内のT細胞特異的転写強化剤である
J M Redondo1, S Hata, C Brocklehurst
1Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115.
まとめ
研究者らは,新しいT細胞受容体 (TCR) デルタ遺伝子増強剤を発見した. この独特の調節要素は,TCRアルファ遺伝子増強剤とは別にTCRデルタ遺伝子転写を制御する.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- T細胞受容体 (TCR) デルタ遺伝子は,TCRアルファロカスの一部である.
- TCR遺伝子の転写調節を理解することは,T細胞の発達と機能にとって極めて重要です.
研究 の 目的:
- TCRデルタ遺伝子発現を制御する規制要素を特定し,特徴づけること.
- TCRデルタとTCRアルファの遺伝子調節の関係を調査する.
主な方法:
- 人間のTCRデルタ遺伝子のJデルタ3-Cデルタイントロン内の新しい転写強化剤の特定.
- Vデルタ1,Vデルタ3および異質プロモーターを用いたレポーターアッセイを用いた強化剤の機能分析.
- 特定の250bpのDNA領域に増強剤の活性をマッピングし,核タンパク質の結合部位を特定します.
主要な成果:
- TCRアルファ増強剤とは異なるT細胞特異増強剤は,TCRデルタ遺伝子で特定されました.
- この増強剤は,TCRデルタプロモーター (Vデルタ1,Vデルタ3) および他のプロモーターからの転写を活性化します.
- 強化剤の活動は,複数の核タンパク質結合部位を持つ250bp領域に存在する.
結論:
- 異なる規制要素は,TCRデルタおよびTCRアルファ遺伝子の転写活動を制御する.
- 特定された増強剤は,TCRデルタ遺伝子発現の調節に重要な役割を果たします.
- この発見は,T細胞受容体遺伝子の複合的な転写制御を理解するのに役立ちます.
さらに関連する動画
11:21Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
Published on: September 10, 2017
11:36An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
Published on: April 21, 2023
関連する概念動画
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
