ドロソフィラのリボソームRNA遺伝子は,雄性メオシス中にX-Yペアリングサイトとして機能する
1Department of Biology, University of Wisconsin, Eau Claire 54701.
Cell
|April 6, 1990
まとめ
リボソームRNA遺伝子は,ドロソフィラのオスにおける性染色体のペアリングを促進する. これらの遺伝子の子宮外挿入は,X-Y染色体のペアリングと分離をメオシス中に強化します.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学とは
背景:
- ドロソフィラ・メラノガスターの雄性における性染色体のペアリングは,精密な半減期に不可欠です.
- このペアリングは,X染色体とY染色体の核組織者を含むヘテロクロマティック領域で通常発生します.
- これらのペアリング領域が欠けているX染色体は,Y染色体からランダムな分離を示します.
研究 の 目的:
- X-Y染色体のペアリングと断絶におけるリボソームRNA (rRNA) 遺伝子の役割を調査する.
- 異胎性rRNA遺伝子の挿入が,X染色体におけるペアリング領域に欠陥のあるペアリング欠陥を救済できるかどうかを判断する.
主な方法:
- P要素媒介変換は,単一のリボソームRNA (rRNA) 遺伝子を異染色体欠陥X染色体に挿入するために使用されました.
- 変形したX染色体とY染色体のペアリングと断絶は,メオシス中に分析されました.
- 自体性rDNA挿入と異胎性rRNA遺伝子の異なる用量のX-Yペアリングに対する効果が評価されました.
主要な成果:
- X染色体への単一のrRNA遺伝子の挿入は,X-Yのペアリングと解離を著しく刺激しました.
- rRNA遺伝子の挿入を伴うX染色体は,挿入部位でペアリングされています.
- オートソームのrDNA挿入は,X-Yペアリングやディズジョンクションに影響を与えなかった.
- Xペアリング部位の強さは,子宮の外部rRNA遺伝子の量と正比であった.
結論:
- リボソームRNA遺伝子は,Drosophila melanogasterにおけるX-Y染色体のペアリングと断絶を促進する能力を持っています.
- 核球組織者は,野生型の雄の自然X-Y交配部として機能する可能性が高い.
- エクトピックrRNA遺伝子は,ネイティブのペアリング領域を機能的に置き換えることができ,中性染色体行動におけるその役割を強調します.
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