SRV2は,酵母におけるアデニル酸サイクラスのRAS活性化に必要な遺伝子です

M Fedor-Chaiken¹, R J Deschenes, J R Broach

¹Department of Biology, Princeton University, New Jersey 08544.

Cell

|April 20, 1990

まとめ

SRV2遺伝子は,RASで活性化されたアデニラートサイクラゼの活動と,細胞の重要な機能に不可欠です. SRV2の突然変異は,ストレス感受性を軽減しますが,その遺伝子が欠けると致死性になります.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

RAS遺伝子経路は,ストレスに対する細胞の反応を調節する.
アデニラートサイクラースの活動は,細胞シグナル伝達に不可欠です.
RAS機能を調節する遺伝子の理解は,細胞生物学にとって重要です.

研究の目的:

活性化されたRASと相互作用する遺伝子を特定する.
細胞のストレス反応とcAMP蓄積におけるSRV2遺伝子の役割を明らかにする.
SRV2タンパク質の機能を特徴付けるために.

主な方法:

活性化されたRASの抑制剤の遺伝子スクリーニング.
遺伝子相互作用を決定するエピスタシス分析.
循環性AMP (cAMP) の蓄積をインビヴォで直接測定する.
タンパク質の機能を特定するための遺伝子配列決定.
ゼロ変異と遺伝抑制剤の分析.

主要な成果:

SRV2遺伝子変異は,活性化されたRAS株におけるストレス感受性を抑制する.
SRV2は,細胞周期性AMP (cAMP) の蓄積に影響する.
SRV2の変異は,RASの活性化時にcAMPの生産を vivoで低下させる.
SRV2のゼロ変異は致死性であり,cAMP依存タンパク質キナーゼの活性化によって抑制されません.
SRV2はアデニラートサイクラゼ関連タンパク質をコードする.

結論:

SRV2タンパク質は,RASで活性化されたアデニラートサイクラースの機能に不可欠です.
SRV2は,cAMPレベルを調節する上で重要な役割を果たします.
SRV2タンパク質は,RASシグナル伝達以外の重要な細胞プロセスに関与しています.

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