チキンのリゾーシムサイレンサーのモジュール構造: 異常な甲状腺ホルモン受容体結合部位の関与
A Baniahmad1, C Steiner, A C Köhne
1Genzentrum, Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Cell
|May 4, 1990
まとめ
増音器のようなサイレンスエレメントは,位置や方向性に関係なく動作します. この研究では,遺伝子活動を抑制し,NeP1や甲状腺ホルモン受容体などの特定のタンパク質と結合するモジュールを持つ鶏のリゾーシムサイレンサーを詳細に説明しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
背景:
- サイレンサー要素は,遺伝子発現を減少させるDNA配列である.
- 機能は,ゲノムの位置と方向性とは関係なく,増強要素と似ています.
研究 の 目的:
- チキンライソジームサイレンサーの特徴を調査する S-2.4 kb.
- このサイレンサーエレメントのモジュール化性質とタンパク質の相互作用を理解するために.
主な方法:
- プロモーターに関連したサイレンサーエレメントの機能の分析.
- 静音器モジュールと相互作用するタンパク質を特定するためのインビトロ結合測定法.
主要な成果:
- チキン・リゾーシム・サイレンサーS-2.4kbは,強化剤のような性質を示しています.
- それは,独立した遺伝子活動を抑制するモジュールで構成され,組み合わせた場合のシナージー効果があります.
- 抑制は,完全なプロモーターと最小のプロモーターの両方で起こります.
- 1つのモジュールは75~93kdのタンパク質 (NeP1) と結合し,もう1つのモジュールは,腫瘍遺伝子のv-erbA産物または甲状腺ホルモン受容体と,異常な逆転パリンドロミック部位で結合する.
結論:
- サイレンサーエレメントは,強化エレメントと機能的な類似性を共有しています.
- 特定されたサイレンサーモジュールは,NeP1および甲状腺ホルモン受容体を含む特定のタンパク質と相互作用し,遺伝子発現に影響を与えます.
関連する概念動画
01:02Protein Digestion
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
02:41Protein Complex Assembly
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
01:30Transducer Mechanism: G Protein–Coupled Receptors
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical, 7TM, or...
GPCRs are also called heptahelical, 7TM, or...
01:27Transducer Mechanism: Enzyme-Linked Receptors
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
01:17The Proteasome Structure
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
The proteasome is an...