ユカリオットのゲノム全体で再構成された核細胞組織のためのパッケージングメカニズム
Zhenhai Zhang1, Christian J Wippo, Megha Wal
1Center for Eukaryotic Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA.
Science (New York, N.Y.)
|May 21, 2011
まとめ
DNA配列ではなく,活性トランス作用因子は,遺伝子開始部位の近くで正規の核細胞配列を確立する. この転写独立メカニズムは,遺伝子調節のための必須クロマチンの組織センターを作り出します.
科学分野:
- 分子生物学は分子生物学である.
- ゲノミクスゲノミクスとは
- クロマチンの生物学
背景:
- ユカリオット遺伝子は,5'端の近くで正規の核群配列を示している.
- 以前の仮説では,この組織が統計的位置付け,DNA配列,または転写開始に起因すると考えられていた.
研究 の 目的:
- ユカリオット遺伝子の5'端の精密な核子の位置付けと距離を制御するメカニズムを調査する.
- 染色体組織センターの形成に起因する要因を特定する.
主な方法:
- 酵母における核細胞配列の生化学的再構成.
- アデノシントリホスファート依存型トランス作用因子の役割を評価する.
- 染色体組織の転写独立メカニズムを評価する.
主要な成果:
- アデノシントライホスファート依存型トランス作用因子は,核細胞の位置づけ,間隔,占有率を成功裏に再構成した.
- これらの要因は,固有のDNAベースのポジショニングを覆す.
- 均一な核細胞間隔は,低密度でも,転写から独立して維持されます.
結論:
- アクティブで非統計的な核細胞パッキングメカニズムは,遺伝子5'端のクロマチンの組織に責任があります.
- このメカニズムは,規制要素の機能に不可欠なクロマチンの組織化センターを確立します.
- DNAでコードされたポジショニングではなく,アクティブな要因が,初期クロマチンの構造を決定する.
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Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
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