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関連する概念動画

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

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関連する実験動画

Updated: Jun 1, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

ヒトの内生性コーレギュレータ複合体の分析

Anna Malovannaya1, Rainer B Lanz, Sung Yun Jung

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA.

Cell
|May 31, 2011
PubMed
まとめ

この研究では,質量スペクトロメトリーを用いてヒトのタンパク質相互作用ネットワークをマッピングし,新しいタンパク質結合と細胞タンパク質の階層化された組織構造を明らかにしました. これは,転写制御の理解のためのリソースを提供します.

さらに関連する動画

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

関連する実験動画

Last Updated: Jun 1, 2026

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
09:33

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

科学分野:

  • プロテオミクス プロテオミクスは,プロテオミクスの
  • システム生物学 システム生物学
  • 分子生物学は分子生物学である.

背景:

  • 固有のタンパク質とタンパク質の相互作用を理解することは,生物学的研究にとって極めて重要です.
  • 既存の方法は,弱い相互作用や一時的な相互作用を捉えるのに失敗することが多い.

研究 の 目的:

  • ヒトの内生的なコレグレータータンパク質複合ネットワークを包括的にマッピングする.
  • 新しいタンパク質の結合を特定し,それらの組織的原理を理解する.

主な方法:

  • 3290の親和性浄化の統合的質量スペクトロメトリーベースの分析.
  • 複雑な隔離中の弱いタンパク質の相互作用の保存.
  • 大量のデータセットで高い相互性を利用する.

主要な成果:

  • 転写ネットワーク (ZMYND8,ZNF687,ZNF592) を含む,報告されていない多数のタンパク質結合の特定.
  • ネットワーク内の階層的な相互作用を明らかにし,プロテオームを最小固有モジュール (MEMO),複合アイソフォーム (unicore) および複合複合相互作用ネットワーク (CCI) に編成しました.

結論:

  • 開発されたリソースは,細胞タンパク質の概念的組織を提供します.
  • 仮説の生成とテストのために,ゲノム研究とタンパク質機能のリンクを容易にする.