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関連する概念動画

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
The DNA Helix01:16

The DNA Helix

Overview
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
The Nucleosome01:19

The Nucleosome

Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...

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関連する実験動画

Updated: May 31, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

DNAの形状的柔軟性について

Andriy Marko1, Vasyl Denysenkov, Dominik Margraf

  • 1Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Str. 7, 60438 Frankfurt, Germany.

Journal of the American Chemical Society
|June 28, 2011
PubMed
まとめ

パルス電子対電子二重共振 (PELDOR) は,二重鎖DNA (ds-DNA) の柔軟性を明らかにしています. ds-DNAの伸縮は,協同的なトウィスト-ストレッチカップリングを誘導し,ヘリックス半径をわずかに減らす.

さらに関連する動画

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

関連する実験動画

Last Updated: May 31, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

科学分野:

  • バイオフィジックス 生物物理学
  • 構造生物学 構造生物学とは
  • 分子ダイナミクス 分子ダイナミクス

背景:

  • 形状の柔軟性は,DNAの機能にとって極めて重要です.
  • DNAのダイナミクスを理解するには,高度なスペクトロスコーピーの技術が必要です.
  • DNAダイナミクスに関する既存のモデルは,実験的検証が必要である.

研究 の 目的:

  • PELDORを用いて,螺旋型のDNAの形状の柔軟性を調査する.
  • ds-DNAの伸縮,回転,曲折の柔軟性を決定する.
  • DNAダイナミクスの様々なモデルを区別する.

主な方法:

  • パルス電子二重共振 (PELDOR) スペクトロスコーピーを利用しました.
  • 固い二酸化窒素のスピンラベルを20塩基対のDNA複合体に組み込みました.
  • XバンドとGバンド (2~4 nm距離) でオリエンテーション選択型PELDOR実験を行った.

主要な成果:

  • 実験データは,ds-DNA.のダイナミックモデルと一致しています.
  • ds-DNAのストレッチは,ツイストと結びついています.
  • この協力的なトウィスト・ストレッチ・カップリングにより,ヘリックス半径がわずかに縮小します.

結論:

  • PELDORはDNAダイナミクスを探査するのに有効です.
  • 協力的なトウィスト・ストレッチ・カップリングメカニズムは,ストレッチに対する ds-DNAの反応を制御する.
  • この発見は,DNAの構造ダイナミクスに関する理解を深める.