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08:01
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
皮膚の発達中のNotchシグナル伝達と表皮の分化におけるプライマリシリウムの役割
Ellen J Ezratty1, Nicole Stokes, Sophia Chai
1Howard Hughes Medical Institute, Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY 10065, USA.
Cell
|June 28, 2011
まとめ
主要なシリアは皮膚の発達に不可欠であり,細胞シグナル伝達と分化を調節する. シリア機能の障害は,ノッチ・シグナル伝達とプロジェンター・エンタメメントを損なっており,皮膚の発達に影響を及ぼします.
科学分野:
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- シリアは,細胞の信号伝達経路において重要な役割を果たします.
- 皮膚の発達と原始細胞の運命における一次シリアの正確な機能は,まだ完全に理解されていません.
研究 の 目的:
- イントラフラゲラ輸送 (IFT) タンパク質とシリアが皮膚の発達における役割を調査する.
- シリア,ノッチ・シグナル伝達,および表皮における祖先の分化との関係を明らかにする.
主な方法:
- shRNA媒介による7つのイントラフラゲラ輸送タンパク質 (IFT) のノックダウン.
- マウスモデルでのIFT-88とKif3aの条件付アブレーション.
- シリア,増殖,ノッチシグナル伝達,およびケラチノサイトと胚性表皮の微分化の分析.
主要な成果:
- ケラチノサイトと胚性表皮におけるシリアの除去は,ほとんどの変異体における過剰増殖につながった.
- シリア変異体は,増殖とは関係なく,ノッチ信号伝達と祖先の分化に欠陥を示した.
- Notchのシグナリングコンポーネントはシリアでコロカライズされ,欠陥は細胞自律であり,活性化されたNotchによって救出されました.
- Shh信号は,表皮のシリオゲネシスや分化には必要なかったが,毛皮のモルフォゲネシスで後に影響を受けた.
結論:
- プライマリーシリアは,皮膚の発達において,異なる時間的,空間的な役割を果たし,信号伝達,増殖,分化に影響を与えます.
- シリアは,表皮におけるノッチ・シグナル伝達とプロジェニター・エンタイメントの重要なレギュレータである.
- Shhシグナル伝達は,皮膚の発達後期,特に毛皮の形態変異に関与しています.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:23Role of Matrix Metalloproteases in Degradation of ECM
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
A...
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:11Papillary Dermis
Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...