関連する実験動画

Updated: May 31, 2026

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
05:52

In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria

Published on: June 28, 2018

生物発光花火のルシフェラゼ-オキシルシフェリンシステムの電子的に興奮状態の表面の動態

Chang-ik Song1, Young Min Rhee

  • 1Institute of Theoretical and Computational Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, 790-784 Korea.

Journal of the American Chemical Society
|July 7, 2011
PubMed
まとめ

の光生成には,ルシフェラーゼ酵素とその基質であるオキシルシフェリンとの複雑な相互作用が伴う. この研究は,水とタンパク質の環境の影響を受けたこれらの相互作用が,放射される光の色をどのように決定するかを明らかにしています.

さらに関連する動画

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)07:04

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

Published on: May 23, 2014

関連する概念動画

Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...