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関連する概念動画

X-Inactivation01:58

X-Inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
X-inactivation01:58

X-inactivation

The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
X and Y Chromosomes02:32

X and Y Chromosomes

Among mammals, the gender of an organism is determined by the sex chromosomes. Humans have two sex chromosomes, X and Y. Every human diploid cell has 22 pairs of autosomes and one pair of sex chromosomes. A human female has two X chromosomes, while a male has one X chromosome and one Y chromosome.
The germline cells such as egg and sperm cells carry only half the number of chromosomes, i.e., 22 autosomes and one sex chromosome. All eggs have an X chromosome, while sperm cells can carry an X or...
Bridge rectifier01:24

Bridge rectifier

The bridge rectifier is essential in electronics for efficiently converting alternating current (AC) to direct current (DC). Comprised of four diodes configured in a bridge layout, this rectifier effectively processes both the positive and negative halves of the AC waveform, making it superior to half-wave and full-wave center-tapped rectifiers in terms of voltage regulation and output stability.
Operationally, the bridge rectifier allows current flow through two of its diodes during each...
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...

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関連する実験動画

Updated: May 31, 2026

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
15:54

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

Published on: June 14, 2014

X不活性化のためのYY1ブリッジ

Joanne L Thorvaldsen1, Jamie R Weaver, Marisa S Bartolomei

  • 1Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 415 Curie Boulevard, Philadelphia, PA 19104, USA.

Cell
|July 7, 2011
PubMed
まとめ

XistRNAは,X染色体と結合することで,X染色体を無活性化する. タンパク質YY1は,XistRNAをDNA結合によって不活性X染色体 (Xi) に結合させ,X染色体の不活性化を促進する.

科学分野:

  • 遺伝学 遺伝学とは
  • 分子生物学は分子生物学である.
  • エピジェネティクス エピジェネティクス

背景:

  • XistRNAは,哺乳類におけるX染色体の不活性化に不可欠である.
  • Xist RNAが不活性X染色体 (Xi) と結合する正確なメカニズムは不明である.

研究 の 目的:

  • 不活性X染色体へのXistRNAをアンカーする分子メカニズムを解明する.
  • Xist RNAとXiの相互作用を媒介するタンパク質を特定する.

主な方法:

  • クロマチン免疫プレシピテーションアッセイ.
  • RNA免疫プレシピテーションアッセイ.
  • タンパク質-RNAとタンパク質-DNAの相互作用の分析.

主要な成果:

  • 転写因子YY1は,Xist RNAの局所化に関与する重要なタンパク質として特定されました.
  • YY1は,Xist RNAと不活性X染色体のDNAの両方に直接結合することが示されました.
  • YY1のこの二重結合能力は,Xist RNAをXiに結びつけるためのメカニズムを提供します.

結論:

さらに関連する動画

DNA Vector-based RNA Interference to Study Gene Function in Cancer
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DNA Vector-based RNA Interference to Study Gene Function in Cancer

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Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
02:44

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay

Published on: December 29, 2023

関連する実験動画

Last Updated: May 31, 2026

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
15:54

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells

Published on: June 14, 2014

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay
02:44

Isolating Interaction-Null/Impaired Mutants Using the Yeast Two-Hybrid Assay

Published on: December 29, 2023

  • YY1は重要な分子ブリッジとして機能し,XistRNAを不活性X染色体に固定します.
  • このアンカリングメカニズムは,X染色体不活性化の開始と維持に不可欠です.
  • この発見は,哺乳類の発達中の遺伝子発現の表遺伝的調節に関する新しい洞察を提供します.