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Updated: May 31, 2026

Chemical Triphosphorylation of Oligonucleotides
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Chemical Triphosphorylation of Oligonucleotides

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シングルサブユニットRNAポリメラーゼによるトランスクリプト開始の反応経路における時間分解のイベント:ラマン結晶学的証拠

Yuanyuan Chen1, Ritwika Basu, Michael L Gleghorn

  • 1Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106, United States.

Journal of the American Chemical Society
|July 13, 2011
PubMed
まとめ

この研究では,ラマン顕微鏡を用いて,N4ファグRNAポリメラーゼ (RNAP) のナクレオチジル伝達反応をリアルタイムで観察した. 発見は,フォスフォディエステル結合形成の重要なステップと,触媒処理中の酵素構成の変化を明らかにしています.

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