ELF4-ELF3-LUX複合体は,日中時計を,日中,下皮細胞の成長の制御と結びつけています
Dmitri A Nusinow1, Anne Helfer, Elizabeth E Hamilton
1Section of Cell & Developmental Biology, Division of Biological Sciences, University of California San Diego 9500 Gilman Drive, La Jolla, California 92093-0130, USA.
Nature
|July 15, 2011
まとめ
ELF3,ELF4,およびLUXタンパク質で構成される夕方複合体は,PIF4およびPIF5発現を制御することによって,植物の成長を調節します. この複合体は,昼間の時計にとって極めて重要です.
科学分野:
- 植物分子生物学 植物分子生物学
- シルカディアン生物学
- アラビドプシス・タリアナの研究
背景:
- 昼間の時計は,植物が環境の変化に適応することを可能にします.
- 光と昼間の時計は,アラビドプシス・タリアナにおけるヒポコチル細胞の延長を同期し,夜明けにピークに達します.
- 日間サイクルで植物の成長を調節する分子メカニズムを理解することは不可欠です.
研究 の 目的:
- ヒポコチル細胞の伸びの昼間調節に責任のあるタンパク質複合体を特定する.
- アラビドプシス・サリアナのこの複合体の分子相互作用と調節機能を明らかにする.
- サーカディアンクロックが光信号に反応して植物の成長をどのようにゲートするかを決定するために.
主な方法:
- 夕方複合体の識別と特徴 (ELF3,ELF4,LUX).
- 昼間の条件下での遺伝子発現パターンの分析.
- タンパク質複合体の局所化と標的遺伝子の調節の調査 in vivo.
- PIF4およびPIF5.5の変異を用いたエピスタシス分析.
主要な成果:
- 夜間複合体は,EARLY FLOWERING 3 (ELF3),ELF4,およびLUX ARRHYTHMO (LUX) で構成されており,植物の成長を直接制御しています.
- 複合体は昼間調節され,夕暮れにピークの豊富さがあります.
- ELF3,ELF4,およびLUXは,成長を促進する転写因子PIF4およびPIF5.5の正しい発現に不可欠です.
- LUXは夕方複合体をPIF4とPIF5のプロモーターに誘導し,PIF4/PIF5の変異は夕方複合体の機能喪失にエピスタティックである.
結論:
- 夕方複合体は,ヒポコチルの成長の昼間ゲートの分子基礎です.
- 夕方複合体によるPIF4とPIF5の調節は,成長反応を媒介する重要な機能である.
- この研究は,昼間の時計と植物成長の調節の間の直接的なリンクを明らかにしています.
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