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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

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Updated: May 30, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

非光学的ゲノムシーケンシングを可能にする統合半導体装置.

Jonathan M Rothberg1, Wolfgang Hinz, Todd M Rearick

  • 1Ion Torrent by Life Technologies, Suite 100, 246 Goose Lane, Guilford, Connecticut 06437, USA. Jonathan.Rothberg@Lifetech.com

Nature
|July 22, 2011
PubMed
まとめ
この要約は機械生成です。

この研究は,スケーラブルで低コストのゲノムシーケンシングのための半導体製造を使用して,新しい非光学的DNAシーケンシング技術を導入しています. イオンチップは,DNA合成の副産物を直接感知し,より速く,より手頃な価格の遺伝子分析を可能にします.

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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

関連する実験動画

Last Updated: May 30, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
09:03

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy

Published on: August 25, 2019

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

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科学分野:

  • バイオテクノロジー バイオテクノロジー
  • ゲノミクスゲノミクスとは
  • 半導体技術は半導体技術である.

背景:

  • DNAシーケンシングは,生命科学,バイオテクノロジー,医学において極めて重要です.
  • 既存の技術は,スケーラビリティとコストの課題に直面しています.
  • より効率的で手頃な価格のシーケンシングソリューションの継続的な需要があります.

研究 の 目的:

  • 新しい非光学的DNAシーケンシング技術について説明します.
  • スケール可能で低コストのゲノムシーケンシングのための半導体製造を活用する.
  • 開発されたイオンチップ技術の性能とスケーラビリティを実証するために.

主な方法:

  • 統合回路製造のための補完的な金属酸化物半導体 (CMOS) プロセスを利用しました.
  • 120万個のイオン感受性フィールド効果トランジスタベースのセンサーを搭載したイオンチップを開発した.
  • 直接的なイオンセンシングのために天然の核酸でテンプレート指向のDNAポリメラーゼ合成を使用しました.
  • バクテリアとヒトゲノムの非光学DNA配列解析を行いました.

主要な成果:

  • イオンチップ技術を使用して,3つのバクテリアゲノムを順序化しました.
  • センサー密度 (10倍まで) を高めたイオンチップを生産することで,堅牢性とスケーラビリティを証明しました.
  • 完全なヒトゲノムの配列を解析し,大規模な応用のためのシステムの能力を示した.
  • DNA合成で生成されるイオンの直接的,非光学的感知を達成した.

結論:

  • 開発された半導体ベースのDNAシーケンシング技術は,スケーラブルで低コストのソリューションを提供します.
  • CMOS製造は,ゲノムシーケンシングのための大規模生産と高密度センサー配列を可能にします.
  • この非光学的アプローチは,ゲノム研究,バイオテクノロジー,医学を前進させる大きな可能性を秘めています.