関連する実験動画
Updated: May 30, 2026
07:27
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
Published on: April 29, 2010
複製チェックポイントは,核孔から転写された遺伝子を放出することによって,フォークの安定性を保護します
Rodrigo Bermejo1, Thelma Capra, Rachel Jossen
1Fondazione Istituto FIRC di Oncologia Molecolare (IFOM) at IFOM-IEO Campus, Via Adamello 16, 20139 Milan, Italy. rodrigo.bermejo@ifom-ieo-campus.it
Cell
|July 26, 2011
まとめ
DNA複製チェックポイントは,遺伝子を核周辺に縛り付ける遺伝子ゲーティングに対抗することによって,DNA複製フォークの崩壊を防ぐ. このプロセスは,トポロジカルな緊張を解放するために,ニュクレオポリンをリン酸化することを含む.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- トランスクリプションは,DNA複製のフォークの進行と安定性を妨げる可能性があります.
- Mec1/ATR (Mec1/ATR) チェックポイント経路は,複製フォークの整合性を維持するために不可欠です.
研究 の 目的:
- 複製フォークの安定性を制御するチェックポイント依存メカニズムを調査する.
- チェックポイント経路が,核周辺の転写から生じるトポロジカルな課題をどのように解決するのかを理解する.
主な方法:
- 特定のヌクレオポリン成分 (THO,TREX-2,inner-basket nucleoporins) が欠けているチェックポイント変異体の分析.
- 遺伝子ゲーティングの役割と,チェックポイントキナーゼによるゲートゲーティングの調節に関する研究.
- 二重鎖断裂アッセイを用いて,Mlp1のリン酸化変異体を分析した.
主要な成果:
- THO,TREX-2,または内部のバスケットのヌクレオポリンが欠けているチェックポイント変異体は,救助されたフォークの逆転と休眠起源の発射を示しています.
- チェックポイントミュータントは,核孔から転写された遺伝子を分離できず,トポロジカル障害を生み出します.
- ダブルスレッドブレイクを導入したり,構成チェックポイントのリン酸化を模倣したりすると,フォークの不安定性が軽減されます.
結論:
- DNA複製チェックポイントは,転写された遺伝子のフォークの進行と安定性を促進します.
- チェックポイントキナーゼは,核孔ゲート遺伝子のトポロジカル緊張を軽減し,核孔ゲート遺伝子のトポロジカル緊張を軽減し,核孔ゲートゲート遺伝子を酸化することによって対抗する.
関連する概念動画
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
02:37Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
01:02The DNA Replication Fork
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
02:36DNA Damage can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
02:36DNA Damage Can Stall the Cell Cycle
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...