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Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Position-effect Variegation02:32

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

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Updated: May 30, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

定量的なエピジェネティックメモリの基礎となるポリコンブベースのスイッチ.

Andrew Angel1, Jie Song, Caroline Dean

  • 1Department of Computational and Systems Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

Nature
|July 26, 2011
PubMed
まとめ
この要約は機械生成です。

ヴァルナライゼーションは,ポリコンブ抑制複合体2 (PRC2) を使用して,ヒストン3リシン27トリメチル化 (H3K27me3) 経由でFLC遺伝子を安定的に静止させます. 寒さへの曝露の期間は,この表遺伝子静止を定量的に制御し,植物の開花に影響を与えます.

さらに関連する動画

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia
07:10

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

Published on: September 15, 2023

関連する実験動画

Last Updated: May 30, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

A Method to Study de novo Formation of Chromatin Domains
07:34

A Method to Study de novo Formation of Chromatin Domains

Published on: August 23, 2019

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia
07:10

Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

Published on: September 15, 2023

科学分野:

  • エピジェネティクスと植物生物学
  • クロマチンの生物学
  • 遺伝子サイレンシングの分子メカニズム

背景:

  • ポリコンブ抑制複合体2 (PRC2) は,H3K27me3.3.を通じて表遺伝的沈黙を確立する.
  • アラビドプシスのヴァルナライゼーションは,PRC2媒介による花の抑制器FLCの静止を伴う.
  • PRC2による静音化の安定性と核化は,依然として重要な研究課題です.

研究 の 目的:

  • ヴァルナライゼーションによるFLCのエピジェネティックサイレンシングの定量的な性質を調査する.
  • FLCロカスにおけるPRC2核形成と安定性のメカニズムを解明する.
  • 寒さへの曝露期間が安定した表遺伝子記憶にどのように影響するかを理解する.

主な方法:

  • エピジェネティック・ダイナミクスの数学モデリング.
  • H3K27me3レベルを定量化するためのクロマチン免疫降水 (ChIP).
  • FLC:GUS レポーターアッセイは,個々の細胞における遺伝子発現パターンを評価するものです.

主要な成果:

  • 寒さへの曝露期間は,安定したH3K27me3媒介のFLCサイレンスを持つ細胞の数を定量的に決定する.
  • H3K27me3のレベルは,冷却処理中にFLC内の局所的な核化領域で徐々に増加します.
  • FLCの場所での二元化可能なエピジェネティック・スイッチングメカニズムが提案されており,寒さの持続時間に依存し,実験的に検証されています.

結論:

  • ヴァルナライゼーションの定量的な側面は,FLCの安定したH3K27me3サイレンシングを達成する細胞の亜集団に依存しています.
  • H3K27me3マークの局所的な核化は,FLC遺伝子発現のバイスタブルスイッチを駆動する.
  • 局所ヒストン改変核化による表皮遺伝的再プログラミングのこのメカニズムは,広く適用される可能性が高い.