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関連する概念動画

Membrane Domains01:18

Membrane Domains

The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
Fluid Mosaic Model01:19

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich with the analogy of...
Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
What are Membranes?01:54

What are Membranes?

A key characteristic of life is the ability to separate the external environment from the internal space. To do this, cells have evolved semi-permeable membranes that regulate the passage of biological molecules. Additionally, the cell membrane defines a cell’s shape and interactions with the external environment. Eukaryotic cell membranes also serve to compartmentalize the internal space into organelles, including the endomembrane structures of the nucleus, endoplasmic reticulum and Golgi...

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関連する実験動画

Updated: May 30, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

空間的分子操作のための細胞膜から派生した連続した脂質二層.

Lisa Simonsson1, Anders Gunnarsson, Patric Wallin

  • 1Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden.

Journal of the American Chemical Society
|July 27, 2011
PubMed
まとめ

研究者は,実際の細胞膜から流体サポートされた脂質バイレイヤー (SLB) を作成する新しい方法を開発しました. この進歩により,先端の細胞膜研究のために,ネイティブの膜成分を効率的に濃縮して分離することが可能になる.

科学分野:

  • バイオフィジックス 生物物理学
  • 細胞生物学 細胞生物学
  • 材料科学 材料科学とは

背景:

  • 複雑な脂質環境におけるネイティブ膜成分の濃縮と分離は,依然として困難です.
  • 実際の細胞膜から連続した,横向的に流体で支えられる脂質二重層 (SLB) を生成するための効率的な方法の欠如は,進歩を妨げています.

研究 の 目的:

  • 本来の細胞膜を含む複雑な脂質組成からサポートされた脂質バイレイヤー (SLB) を生成するための効率的な方法を開発する.
  • これらのSLBs内のネイティブ膜成分の移転と保存された横向移動性を実証する.

主な方法:

  • 水力動力学的に駆動されたSLBのエッジを利用して,吸収された脂質小胞の破裂を誘導しました.
  • 3T3 線維芽細胞の細胞膜から直接派生した膀を備えた,溶融したプリフォームド SLBs.
  • コレラ毒素Bサブユニット (CTB) を用いてギャングリオシド受容体 (G(M1) とG(M3) に結合する分子移転を検証し,水力動力学的流れによる横向移動性を評価した.

主要な成果:

  • 複雑な脂質ベジクルとネイティブ細胞膜からSLBsに膜成分を成功裏に転送しました.
  • SLB内の移転したギャングリオシド (G(M1) / G(M3) の横向移動性の保存が実証されています.

さらに関連する動画

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

関連する実験動画

Last Updated: May 30, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

Published on: December 1, 2015

  • 異なるギャングリオサイドアンカー番号と相関するCTB結合の2つの異なる集団を特定しました.

    • 結論:

    • 水力力学的に駆動されたSLBのエッジは,膀の破裂を効率的に誘導し,ネイティブの膜成分を転送することができます.
    • この方法は,複雑な脂質組成から液体SLBの生成を容易にし,ネイティブ膜の組織と動態の研究を可能にします.
    • この技術は,特定の膜タンパク質と脂質の濃縮と分離のための新しいアプローチを提供します.