Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
Transcytosis of IgG01:15

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Layer-number-parity-dependent abnormal magnetic ordering in few-layer CrI3 on N-face AlN substrate.

Nature communications·2026
Same author

APOE Isoform-Dependent Self-Association Measured by a Split-Luciferase Complementation Assay: Differential Effects of Disease-Risk and Protective Variants.

medRxiv : the preprint server for health sciences·2026
Same author

Development and validation of artificial intelligence-based model for bladder cancer immunophenotyping using whole slide images.

NPJ precision oncology·2026
Same author

CXCR4 antagonism corrects neutrophil abnormalities and reduces pneumonia severity in a pharmacological mouse model of CXCR2 loss-of-function-mediated neutropenia.

Frontiers in immunology·2025
Same author

Wafer Scale III-Nitride Deep-Ultraviolet Vertical-Cavity Surface-Emitting Lasers Featuring Nanometer-Class Control of Cavity Length.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

GPR25 promotes the formation of lung and liver tissue-resident memory CD8 T cells.

Science immunology·2025
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: May 30, 2026

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

オキシステロールは,EBI2を通じてB細胞の移動を直接導きます.

Changlu Liu1, Xia V Yang, Jiejun Wu

  • 1Johnson & Johnson Pharmaceutical Research & Development, L.L.C., 3210 Merryfield Row, San Diego, California 92121, USA. cliu9@its.jnj.com

Nature
|July 29, 2011
PubMed
まとめ
この要約は機械生成です。

オキシステロールは,7α,25-ジヒドロキシコレステロールと同様に,EBI2受容体の内生リガンドとして識別され,B細胞の移動と免疫応答を制御します. この発見は,免疫系におけるオキシステロールの新たな役割を明らかにしています.

さらに関連する動画

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
09:32

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting

Published on: September 19, 2025

関連する実験動画

Last Updated: May 30, 2026

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
08:22

Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production

Published on: May 31, 2020

Detection of True IgE-expressing Mouse B Lineage Cells
09:40

Detection of True IgE-expressing Mouse B Lineage Cells

Published on: December 1, 2014

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting
09:32

Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting

Published on: September 19, 2025

科学分野:

  • 免疫学 免疫学とは
  • 分子生物学は分子生物学である.
  • コレステロールの代謝

背景:

  • EBI2 (GPR183) は,B細胞の移動と抗体生成に不可欠な孤児Gタンパク質結合受容体である.
  • オキシステロールは,主に核ホルモン受容体を通して,免疫反応とコレステロール代謝に影響を与えます.

研究 の 目的:

  • EBIの内生リガンドを特定するために2.2.
  • EBI2媒介の免疫機能におけるオキシステロールの役割を明らかにする.

主な方法:

  • 豚ののエキスからオキシステロールを分離する.
  • EBI2 を発現する細胞と欠乏細胞を用いた in vitro 移行アッセイ.
  • EBI2欠乏したマウスとCYP7B1阻害を対象としたインビボ試験.

主要な成果:

  • 7α,25-ジヒドロキシコレステロール (OHC) は,EBI2 (Kd = 450 pM) の強力な内生リガンドとして特定されました.
  • 7α,25-OHCは,B細胞とT細胞のEBI2に依存した移動をインビトロで刺激する.
  • In vivoでは,EBI2欠乏症または7α,25-OHC無敏感化は,B細胞が胞の葉っぱに寄与することを損なう.

結論:

  • EBI2とオキシステロール,特に7α,25-OHC.との間の直接的なリンクを確立します.
  • EBI2媒介の免疫細胞の密輸におけるシグナル伝達分子としてのオキシステロールの新たな役割を明らかにした.
  • 免疫反応とB細胞の局所化をリンパ性臓器内で調節する新しい経路を強調しています.