siRNAマイクロヒドロゲルによる遺伝子サイレンシングは,ナノスケールのポリマー濃縮によるものです
Cheol Am Hong1, Soo Hyeon Lee, Jee Seon Kim
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, South Korea.
Journal of the American Chemical Society
|August 12, 2011
まとめ
研究者らは,効率的な遺伝子静止のために,生物学的に活性な小型の干渉RNA (siRNA) ベースのマイクロヒドロゲルを開発した. これらの新しいナノ粒子は,高い細胞吸収と強力な遺伝子静止活性を示しています.
科学分野:
- バイオテクノロジー バイオテクノロジー
- ナノテクノロジー ナノテクノロジー
- 分子生物学は分子生物学である.
背景:
- 小型の干渉RNA (siRNA) は,遺伝子サイレンシングの重要なツールです.
- siRNAの安定的かつ効果的な配送システムを開発することは,治療用途において極めて重要です.
- 以前の方法は,安定性や細胞吸収に関する課題に直面することが多い.
研究 の 目的:
- 生物学的に活性なsiRNAベースのマイクロヒドロゲルの最初の製剤について報告する.
- siRNAマイクロヒドロゲルの自己組み立てとナノ粒子形成を調査する.
- 製造されたナノ粒子の細胞吸収効率と遺伝子静止活性を評価するために.
主な方法:
- 単一鎖の線形およびY形トリメア siRNAsのセンセス/アンティセンセスの補完的なハイブリダイゼーションを使用して自己組み立てによるマイクロヒドロゲルの製造.
- カチオンのポリマー,LPEI (ポリエチレンアミネ) を使用してsiRNAマイクロヒドロゲルの凝縮.
- その結果,コンパクトで安定したsiRNA/ポリマーナノ粒子の特徴.
主要な成果:
- 生物学的に活性なsiRNAベースのマイクロヒドロゲルを成功裏に準備しました.
- ハイブリダイゼーションとポリマー凝縮による自己組み立てとナノ粒子形成が実証されています.
- siRNA/ポリマーナノ粒子の優れた細胞吸収効率を達成しました.
- 細胞モデルで重要な遺伝子静止活性を示した.
結論:
- 開発されたsiRNAマイクロヒドロゲルは,遺伝子配送のための新しい効果的なプラットフォームを表しています.
- カチオンのポリマー凝縮と組み合わせた自己組み立てアプローチは,優れた生物学的性能を持つ安定したナノ粒子を生成します.
- この技術は,RNA干渉ベースの治療法を進歩させるのに有望である.
関連する概念動画
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:30Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...