人間の精子の結合は,シアリル-ルイス (sialyl-Lewis) オリゴサッカリドによって,ゾナ・ペルルシダ (zona pellucida) に媒介される
Poh-Choo Pang1, Philip C N Chiu, Cheuk-Lun Lee
1Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, SW7 2AZ, UK.
まとめ
シアリル-ルイス (x) 炭水化物配列は,精子に対するヒトのゾナ・ペルシダ (ZP) の主要な結合部位である. この発見は,人間の受精と精子と卵の結合における重要なステップを明確にします.
科学分野:
- 生殖生物学 生殖生物学
- グライコバイオロジーは,
- 細胞の粘着は,細胞の粘着です.
背景:
- 人間の受精は,精子が卵細胞を取り囲む細胞外マトリックスであるZona pellucida (ZP) に結合することを意味します.
- ZPの炭水化物配列は精子の結合に作用することが知られているが,特定のリガンドは特定されなかった.
研究 の 目的:
- 精子がヒトの透明ゾーン (ZP) に結合する主な炭水化物リガンドを特定する.
主な方法:
- 超敏感質量スペクトロメトリを用いて,ヒトZPの末端グリカン配列を分析した.
- 機能的測定は,シアリル-ルイス (sialyl-Lewis) 結末型グリココンジュガートおよび特定の抗体を使用して精子-ZP結合を阻害することを含む.
主要な成果:
- シアリル-ルイス (sialyl-Lewis) 配列[NeuAcα2-3Galβ1-4(Fucα1-3) GlcNAc]は,ヒトZP N-およびO-グリカンで最も豊富に存在する末端グリカンとして特定されました.
- 精子-ZP結合は,シアリル-ルイス (sialyl-Lewis) 配列を標的としたグリココンジュガートと抗体によって著しく抑制された.
結論:
- シアリル-ルイス (sialyl-Lewis) 配列は,人間の精子-卵の結合をゾナ・ペルシダに媒介する主要な炭水化物リガンドである.
- この発見は,ヒト受精の分子メカニズムに関する重要な洞察を提供します.
関連する概念動画
Spermatogenesis
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...
Spermatogenesis
Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
Sperm Structure and Semen Composition
During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
Sperm Transport
The journey of sperm from its origin to the point of ejaculation begins within the seminiferous tubules of the testis. Here, Sertoli cells produce fluid that propels non-motile sperm through a series of conduits, starting with the straight tubules leading to the rete testis. This interconnected network of tubules acts as the initial pathway for sperm, guiding them into the efferent ductules and then into the epididymis for maturation.
The maturation phase occurs in the epididymis, where sperm...
The maturation phase occurs in the epididymis, where sperm...
Yeast Signaling
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...


