核プロモーター因子,TAF3による胚性幹細胞系統のコミットメントの制御
Zhe Liu1, Devin R Scannell, Michael B Eisen
1Howard Hughes Medical Institute, Molecular and Cell Biology Department, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|September 3, 2011
まとめ
TAF3は,コアプロモーター因子であり,胚性幹細胞の多能性にとって極めて重要です. 系統の差別化とクロマチンの相互作用を調節し,発達プログラムを保護します.
科学分野:
- 発達生物学 発達生物学について
- 幹細胞生物学 幹細胞生物学
- 分子遺伝学 分子遺伝学
背景:
- 多能性を理解することは,発達と胚性幹細胞 (ESC) 研究の鍵です.
- プラリポテンスを維持する分子機構は複雑で,完全に理解されていない.
研究 の 目的:
- ESCにおけるTBP関連因子であるTAF3の役割を調査する.
- 系統の差別化とクロマチンの調節におけるTAF3の機能を明らかにする.
主な方法:
- TAF3の局所化をマッピングするための全ゲノム結合研究.
- TAF3のCTCFとコヘシンとの相互作用の分析.
- エンドダーム,ニューロエクトダーム,メソダームの分化におけるTAF3の役割の評価.
主要な成果:
- TAF3はESCに非常に富んでおり,内皮の分化に不可欠です.
- TAF3は,早めのニューロエクトダームとメソダームの特異化を防ぐ.
- TAF3はCTCF/コヘシン関連領域に結合し,DNAループを媒介する.
結論:
- TAF3は,長距離クロマチンの相互作用を調節する新しい役割を果たしています.
- これらの相互作用は,多能性の転写バランスを維持するために重要である.
- TAF3は,ESCのアイデンティティと差別化可能性を保護する重要な規制役として機能します.
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