Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

DNA hypomethylating agents preserve T cell stemness and potentiate the efficacy of CD3-bispecific antibodies.

Cancer immunology research·2026
Same author

Multi-Omics and Machine Learning Integration Identifies Key Endothelial Modules in Carotid Artery Stenosis.

Journal of clinical laboratory analysis·2026
Same author

Physiologic and anesthetic predictors of false-positive motor-evoked potential alerts during pediatric spine surgery.

Spine deformity·2026
Same author

HEB collaborates with TCR signaling to upregulate <i>Id3</i> and enable γδT17 cell maturation in the fetal thymus.

eLife·2026
Same author

Targeting CRTC2 reverses <i>STK11</i> mutant NSCLC tumor resistance to immunotherapy.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Single-Cell Profiling Identifies CLEC5A+ Macrophages as Key Drivers of Thoracic Aortic Aneurysm Via CCL5-Mediated M1 Polarization.

Journal of clinical laboratory analysis·2026

関連する実験動画

Updated: May 29, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

CTCFの結合要素は,V(D) J再結合の制御を媒介する.

Chunguang Guo1, Hye Suk Yoon, Andrew Franklin

  • 1Howard Hughes Medical Institute, The Children's Hospital, The Immune Disease Institute, Department of Genetics, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.

Nature
|September 13, 2011
PubMed
まとめ

研究者らは,マウスにおける免疫グロブリン重鎖 (IgH) V(D) J再結合に不可欠な新しい調節領域であるIGCR1を特定した. この領域は,遺伝子セグメントの再編成を制御することによって,適切なB細胞発育と抗体の多様性を確保します.

さらに関連する動画

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

関連する実験動画

Last Updated: May 29, 2026

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

科学分野:

  • 免疫学 免疫学とは
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 発達中のBリンパ球は,V,D,J,Hの遺伝子セグメントから免疫グロブリン重鎖 (IgH) 変異領域エクソンを組み立てます.
  • 2.7メガベースマウスのIghロクスは,特定で多様な抗体レパートリーのためにV(D) J再結合の正確な調節を必要とします.

研究 の 目的:

  • マウスのIghロカス内の新しい調節領域を特定し,特徴づけ,V(D) J再結合に関与する.
  • この領域がB細胞の発達と抗体レパートリー形成における機能的役割を明らかにする.

主な方法:

  • ネズミのV (H) とDの遺伝子クラスターの間のインタージェニックコントロール領域1 (IGCR1) の識別.
  • CTCFのループ/断絶因子結合要素を用いた機能分析と,遠隔強化器を用いたIGHループのメディエーション.

主要な成果:

  • IGCR1は,D (H) -近辺V (H) ゲンのセグメントの転写と再編成を抑制し,遠端V (H) ゲンのセグメントの再編成を促進します.
  • IGCR1は,チモサイトにおける不適切な結合を防止し,フィードバックを調節することにより,秩序あるV (H) (D) (J) (H) リコンビネーションを保証する.
  • IGCR1は,フィードバックの調節と近辺V(H) からDJ(H) の再結合のアレル排除に不可欠です.

結論:

  • IGCR1は,免疫グロブリン重鎖V(D) J再結合のための重要な規制領域である.
  • IGCR1は,正常なB細胞発育,抗体レパートリーバランス,および系統固有の再結合において重要な役割を果たします.
  • この研究は,CTCFタンパク質がIghの位置を調節する新しい機能を強調しています.