キラーとの互換性は,RNAi欠乏菌の出現を説明する
Ines A Drinnenberg1, Gerald R Fink, David P Bartel
1Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA.
まとめ
RNA干渉 (RNAi) 経路について
科学分野:
- 分子生物学は分子生物学である.
- 菌類学 菌類学とは
- 進化生物学の進化生物学について
背景:
- RNA干渉 (RNAi) 経路は,真核生物におけるウイルスとトランポゾンに対する保存された防御機構である.
- RNAiは,Saccharomyces cerevisiaeなどのいくつかの真核系に顕著に欠けています.
- 二重鎖RNA (dsRNA) キラーウイルスは,真菌に多く見られ,毒素生成などの恩恵を与える.
研究 の 目的:
- RNAi経路と真菌におけるdsRNAキラーウイルスの存在の間の進化的関係を調査する.
- なぜ一部の真菌種にRNAi経路が欠けているのかを理解するために.
主な方法:
- Saccharomyces cerevisiaeにおけるRNAi経路の再構成について.
- RNAiとキラーウイルスの有無を比較した真菌種の比較分析.
主要な成果:
- S. cerevisiaeにRNAiを再導入することで,その有益なdsRNAキラーウイルスが失われました.
- 菌類の間でRNAiとdsRNAキラーウイルスの存在の間に強い負の相関が観察されました.
- 致死ウイルスを持つ真菌種は一貫してRNAiが欠け,RNAiを持つ関連種は致死ウイルスが欠けていた.
結論:
- RNAiとdsRNAキラーウイルスは互換性がなく,RNAi機構とのウイルス干渉による可能性が高い.
- 殺人ウイルスによる恩恵は選択的優位性をもたらし,RNAi欠乏菌株の進化的持続性を説明する.
- 特定の真菌におけるRNAiの欠如は,有益なウイルス感染症を維持するための進化的適応である.
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