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関連する概念動画

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

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関連する実験動画

Updated: May 29, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

RNAiのためのヌクレオシド最適化:高通量プラットフォーム

Gabor Butora1, Denise M Kenski, Abby J Cooper

  • 1Department of Process Chemistry, Merck & Co., Rahway, New Jersey 07065, USA. gabor_butora@merck.com

Journal of the American Chemical Society
|September 28, 2011
PubMed
まとめ
この要約は機械生成です。

研究者は,siRNAの安定性と治療の可能性を高めるために,RNA誘発サイレンシング複合体 (RISC) のガイドストランド (GS) の骨幹を修正した. この体系的なアプローチは,強力で安全で効果的なRNA干渉治療法の合理的な設計を可能にします.

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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

関連する実験動画

Last Updated: May 29, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

科学分野:

  • 分子生物学は分子生物学である.
  • RNA干渉療法 治療薬
  • ドラッグ・ディスカバリー・ディスカバリー・ドラッグ・ディスカバリー・ドラッグ・ディスカバリー

背景:

  • RNA誘発サイレンシング複合体 (RISC) は,配列特異的なmRNA分裂のためにガイドストランド (GS) を利用します.
  • GSバックボーンを修正することで,RISCの活動を調節し,siRNAの性質を改善する戦略が提供されます.

研究 の 目的:

  • RISCのGS内の改変核酸を体系的に評価する.
  • 改善された小干渉RNA (siRNA) の合理的な設計のためのプラットフォームを確立する.

主な方法:

  • 修飾された4つのカノニカル塩基すべてのためのフォスフォラミド酸塩の合成.
  • 21核酸GSの各位置における改変核酸の配列評価.
  • 糖改変核酸を比較するためのベースラインとしてイノシンを使用した.
  • 特定の位置で2'-O-ベンジル改変を使用してプラットフォームを検証しました.

主要な成果:

  • GSの5位,8位,15位,19位は,2'-O-ベンジルなどの大容量の改変に対応できることを実証しました.
  • 改変核酸化物を評価するための高スループットメソドロジーを確立しました.
  • イノシン置換を用いた新しい活性ベースラインを定義しました.

結論:

  • 開発されたハイ・スループット・メソドロジーは,siRNAsの仮説主導の設計を容易にする.
  • 治療目的で強力で,免疫学的に静かで,安定したsiRNAsの生成を可能にします.
  • 次世代のRNA干渉ベースの治療法の開発を進めています.