ガンマチューブリン (Gamma-tubulin) は,アスペルギルス・ニドゥランス (Aspergillus nidulans) のマイクロチューブルの機能に不可欠なスパインドルポールボディの成分です
B R Oakley1, C E Oakley, Y Yoon
1Department of Molecular Genetics, Ohio State University, Colombus 43210.
Cell
|June 29, 1990
まとめ
Gamma-tubulinは,A. nidulansの mipA遺伝子によってコードされ,マイクロチューブル機能と核分裂に不可欠です. mipAの変異は致命的で,細胞分裂を阻害し,微小管組織に欠陥を引き起こす.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- チューブリンタンパク質は,細胞構造と分裂に不可欠なマイクロチューブルを形成します.
- ガンマチューブリン (Gamma-tubulin) は,未知の in vivo 機能を持つチューブリンスーパーファミリーの最近特定されたメンバーです.
研究 の 目的:
- アスペルギルス・ニドゥランスにおけるガンマチューブリンのインビボ機能を調査する.
- ガンマチューブリンをコードする mipA 遺伝子の機能喪失変異によって引き起こされる細胞欠陥の特徴を特定するために.
主な方法:
- 統合的変換を使用して mipA 遺伝子変異を生成しました.
- ヘテロカリオンにおける突然変異を維持し,胞子におけるその現象型を研究した.
- 免疫光顕微鏡を用いて,微小管の組織とスパインドルのポールボディを分析した.
主要な成果:
- mipA変異は致命的で後退性であり,核分裂を厳しく抑制する.
- 核の移動と細胞プラズマ微小管の組織も影響を受けた.
- 免疫光検査は,サイトプラズマ微小管の減少とミトック装置の欠如を明らかにし,ガマチューブリンがポールの体に局所されている.
結論:
- ガンマチューブリンは,一般的なマイクロチューブルの機能,特に核分裂に不可欠です.
- ガンマチューブリンは,ミクロチューブルをスピンドルポールボディに結合させ,ミクロチューブルのアセンブリを核化し,ミクロチューブルの極性を確立する役割を果たしている可能性が高い.
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