Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Oxidation of Phenols to Quinones01:17

Oxidation of Phenols to Quinones

In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox property is crucial in...
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
Oxidation-Reduction Reactions03:11

Oxidation-Reduction Reactions

Oxidation–Reduction Reactions
Reactivity of Enols01:18

Reactivity of Enols

Enols are a class of compounds where a hydroxyl group is attached to a carbon–carbon double bond, which implies that it is a vinyl alcohol. A carbonyl compound with an α hydrogen undergoes keto–enol tautomerism and remains in equilibrium with its tautomer, the enol form. Usually, the keto tautomer is present in a higher concentration than the enol tautomer due to the higher bond energy of C=O compared to C=C. Moreover, the direction of the keto–enol equilibrium is governed by factors like...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Redox Reactions01:24

Redox Reactions

Oxidation-reduction or redox reactions involve the transfer of electrons from one molecule or atom to another. When an atom gains an electron, another atom must lose an electron, meaning oxidation and reduction must occur together. Since the redox occurs in pairs, the atom that gets oxidized is also called the reducing agent or reductant, and the atom that is reduced is also called the oxidizing agent or oxidant. A straightforward way to remember the definitions of oxidation and reduction is...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Structural and biochemical basis for cannabinoid cyclase activity in marine bacterial flavoenzymes.

Nature chemical biology·2026
Same author

Lens aging and disease: Molecular mechanisms, functional consequences, and pharmacological implications.

Progress in retinal and eye research·2026
Same author

Consumption of processed foods impairs memory function through dietary advanced glycation end-products.

bioRxiv : the preprint server for biology·2026
Same author

Structural and biochemical basis for cannabinoid cyclase activity in marine bacterial flavoenzymes.

bioRxiv : the preprint server for biology·2025
Same author

Diabetes Endothelial Keratoplasty Study: Methods and Impact on the Use of Corneas From Donors With Diabetes for Descemet Membrane Endothelial Keratoplasty.

Cornea·2025
Same author

Prevention of age-related truncation of γ-glutamylcysteine ligase catalytic subunit (GCLC) delays cataract formation.

Science advances·2024

関連する実験動画

Updated: May 29, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
12:08

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

フラボ酵素における酸素反応性:文脈的な問題

Claudia A McDonald1, Rebecca L Fagan, François Collard

  • 1Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-0606, USA.

Journal of the American Chemical Society
|October 1, 2011
PubMed
まとめ

フラビン共因子を含む酵素は,酸素と素早く反応する. プラスの電荷がフラビンの近くにある.

科学分野:

  • バイオケミストリー バイオケミストリー
  • 酵素学 酵素学とは
  • バイオ・オーガニック化学 バイオ・オーガニック化学

背景:

  • オキシダゼとモノオキシゲナゼを含むフラボ酵素は,フリーフラビンと比較して,酸素との反応がより速い.
  • 減少したフラビンのN5位置に近い陽性電荷の役割は,この強化された反応性の重要な要因として提案されています.
  • これらのメカニズムを理解することは,酵素の機能と進化の解明に不可欠です.

研究 の 目的:

  • フラボ酵素による酸素活性化におけるフラビンN5の近くの陽性電荷のライシン残留物の役割を調査する.
  • ワイルドタイプのフルクトオサミン酸化酵素とダイヒドロロート酸脱水酸化酵素の酸素反応性を,それらのライシン変異体と比較する.
  • 陽性電荷だけでは酸素の反応性を高めるのに十分であるか,または特定の酵素の文脈が必要かどうかを判断する.

主な方法:

  • サイト・ディレクテッド・ミュータジェネシスは,フルークトサミンの酸化酵素でライシンからメチオニンへの変異体 (Lys276Met) を生成するために使用されました.
  • 野生型および変異性酵素の酸素との反応の速度定数を測定するために,運動測定を行った.
  • E. coli と L. lactis のフルークトサミン酸化酵素とダイヒドロロロテート脱水酸化酵素に対する酸素反応速度の比較分析が行われました.

主要な成果:

さらに関連する動画

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

関連する実験動画

Last Updated: May 29, 2026

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
12:08

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
10:14

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase

Published on: November 8, 2019

  • フルクトサミン酸化酵素は,酸素反応の高速常数 (1.6 × 10^5 M^-1 s^-1) を示したが,これはLys276Met変異体 (291 M^-1 s^-1) で劇的に低下し,ライシンの重要性を示している.
  • また,E. coliおよびL. lactisからのダイヒドロロ酸塩酸脱水酵素は,重要な酸素反応速度 (それぞれ6.2 × 10^4および3.0 × 10^3 M^-1 s^-1) を示した.
  • しかし,それらの対応するライシン変異体 (Lys66MetとLys43Met) は,野生型酵素に似た速度定数を示し,文脈に依存する機能を示唆しています.

結論:

  • フラビンN5の近くにあるライシン残留物の存在だけでは,酸素反応性の強化を保証するには不十分です.
  • 特定の酵素環境または"文脈"は,このライシンが酸素活性化をどのように促進するかに重要な役割を果たします.
  • 進化は,さまざまな酵素で,フラビンと酸素の急速な反応を達成するために,さまざまな戦略を採用しています.