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関連する概念動画

DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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関連する実験動画

Updated: May 28, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

TALエフェクター:DNAターゲティングのためのカスタマイズ可能なタンパク質.

Adam J Bogdanove1, Daniel F Voytas

  • 1Department of Plant Pathology, 351 Bessey Hall, Iowa State University, Ames, IA 50011, USA. ajbog@iastate.edu

Science (New York, N.Y.)
|October 1, 2011
PubMed
まとめ

トランスクリプションアクティベーターのようなエフェクターは,DNA配列を正確にターゲットにするための新しい方法を提供します. このブレークスルーにより,研究と医学における多様な応用のための正確な遺伝子編集と操作が可能になります.

科学分野:

  • ゲノミクスゲノミクスとは
  • 分子生物学は分子生物学である.
  • 合成生物学 合成生物学とは

背景:

  • 生体細胞における нуклеотиド配列と遺伝子発現の標的型変異は,ゲノム研究を前進させる上で極めて重要であるが,依然として大きな課題である.
  • 遺伝子変異または転写操作のためにタンパク質を誘導するためにDNA結合ドメインを設計する既存の方法は,しばしば必要な特異性がない.

研究 の 目的:

  • トランスクリプションアクティベーター型 (TAL) エフェクタを高度に特異的なDNAターゲティングシステムとして導入し,その有用性を探求する.
  • 遺伝子操作における特異性の課題を克服するために,TALエフェクタのモジュラーDNA結合領域を強調する.

主な方法:

  • TALエフェクタのモジュラーDNA結合ドメインを活用し,タンデム,ポリモルフのアミノ酸の繰り返しを含む.
  • TALエフェクタドメインの各重複は,DNAの連続した核酸を指定し,正確な配列認識を可能にします.

主要な成果:

  • TALエフェクターには,モジュラーな重複構造があるため,DNAターゲティングにおける高度の特異性があります.
  • TALエフェクタシステムは,正確なDNA配列操作を達成する上で以前の制限を効果的に克服しています.

結論:

さらに関連する動画

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

関連する実験動画

Last Updated: May 28, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
11:24

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains

Published on: March 25, 2015

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
09:56

Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins

Published on: October 31, 2025

  • TALエフェクタ DNA結合ドメインは,標的型遺伝的アプリケーションのための強力なツールを提供します.
  • この技術は,遺伝子機能の研究,作物改良,遺伝疾患の治療開発など,幅広い可能性を秘めています.