モジュラー型クリン-RINGリガゼ基板のグローバル識別
Michael J Emanuele1, Andrew E H Elia, Qikai Xu
1Division of Genetics, Brigham and Women's Hospital, Boston, MA 02115, USA.
Cell
|October 4, 2011
まとめ
Cullin-RING連鎖酵素 (CRL) は,NUSAP1.1のような既知のおよび新しい基板を含む何百ものタンパク質を調節する. これは,CRLの全域的存在を明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- クリン・リング・リガゼ (Cullin-RING ligases,CRLs) は,真核生物における最大のE3ユビキチンリガゼファミリーである.
- CRLの基質を特定することは,プロテオーム調節を理解するために非常に重要です.
研究 の 目的:
- CRLの基質を特定し,細胞プロセスにおけるその役割を理解する.
- CRLによるNUSAP1の規制を調査する.
主な方法:
- 遺伝的および薬学的カルリン不活性化.
- 遺伝子 (GPS) とプロテオミック (QUAINT) アッセイ.
- タンパク質の安定性および無所不在性の分析.
主要な成果:
- 既知のおよび新しい基板を含む数百のCRL規制のタンパク質が特定されました.
- NUSAP1は,細胞サイクル (SとG2) とDNA損傷の間にSCF ((Cyclin F)) 基質として特定されました.
- 調節された基板は,タンパク質の相互作用ネットワークに富んでいる.
結論:
- CRLのユビキティレーションは,細胞生物学において広範な役割を果たします.
- 特定された基底は,細胞生理学の重要な指標を提供します.
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