候補タンパク質キナーゼC遺伝子であるPKC1は,S. cerevisiaeの細胞サイクルに必要なものです
D E Levin1, F O Fields, R Kunisawa
1Department of Microbiology and Immunology, University of California, San Francisco 94143.
Cell
|July 27, 1990
まとめ
芽生える酵母Saccharomyces cerevisiaeは,細胞分裂に不可欠なPKC1という遺伝子を持っています. この遺伝子の削除は,DNA複製後の細胞サイクル停止を引き起こし,PKC1.1によって調節される新しい細胞サイクルチェックポイントを示します.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- タンパク質キナーゼC (PKC) イゾ酵素は,哺乳類の細胞における重要な調節因子である.
- 酵母のような下層エウカリオットのPKCホモログの機能はよく理解されていません.
研究 の 目的:
- 哺乳類のタンパク質キナーゼCに関連したSaccharomyces cerevisiaeの遺伝子を特定し,特徴づけること.
- 細胞周期調節における特定された酵母PKC遺伝子の役割を調査する.
主な方法:
- ネズミのPKC同酵素を検出するためのcDNAプローブを使用してSaccharomyces cerevisiaeゲノムのスクリーニング.
- PKC1喪失のフェノタイプ効果を分析するための遺伝子消去および枯渇の研究.
- PKC1 欠乏した細胞の細胞サイクル分析.
主要な成果:
- 哺乳類のPKCアルファ,ベータ,ガンマ亜種に同類する1つの酵母遺伝子,PKC1が分離されました.
- PKC1の削除は,後退性致死性をもたらしました.
- PKC1が枯渇した細胞は,細胞分裂サイクル (cdc) 変異フェノタイプを示し,DNA複製後の複製を停止し,小さな芽でミトーシスの前に,新しいチェックポイントを示唆しました.
結論:
- Saccharomyces cerevisiaeのPKC1遺伝子産物は,細胞の生存に不可欠であり,以前に認識されていない細胞サイクルチェックポイントを調節します.
- この発見は,Eukaryotesの細胞サイクル制御におけるPKCシグナル伝達経路の保存された役割を示唆しています.
関連する概念動画
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M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...