Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
X-ray Crystallography02:18

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
Protein Folding01:22

Protein Folding

Overview
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Determination of Crystal Structures01:29

Determination of Crystal Structures

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Hierarchical learning creates invariant schema within plastic neural networks.

Journal of computational neuroscience·2026
Same author

Saturation mutagenesis map of generalist versus specialist adaptations of β-lactamase to novel antibiotics.

bioRxiv : the preprint server for biology·2026
Same author

Mechanism of MEK1 activation by phosphorylation.

Biophysical journal·2026
Same author

Discrete protein dynamics enable long-range communication.

bioRxiv : the preprint server for biology·2025
Same author

Ultrafast Dynamics of Porphyrins in the Condensed Phase:  II. Zinc Tetraphenylporphyrin<sup>†</sup>.

The journal of physical chemistry. A·2025
Same author

Many will enter, few will win: Cost and sensitivity of exploratory dynamics.

Biophysical journal·2025
Same journal

Carbonylative Aminative Suzuki-Miyaura Coupling: Pd-Catalyzed Synthesis of Amides from Vinyl/Aryl Halides and Boronic Acids.

Journal of the American Chemical Society·2026
Same journal

Divergent Asymmetric Synthesis of Glutinosasins A-E.

Journal of the American Chemical Society·2026
Same journal

Ultrastrong Polyketone Hot-Melt Adhesives Enabled by Ni-Catalyzed Carbonylative Polymerization.

Journal of the American Chemical Society·2026
Same journal

Programmable Anomalous Photovoltaics Enabled by Light-Electric Dual-Field Control.

Journal of the American Chemical Society·2026
Same journal

Biomimetic Redox-Mediated Proton Relay in Nanoreactors for Photocatalysis.

Journal of the American Chemical Society·2026
Same journal

The Sulfur Monoxide-Water Complex.

Journal of the American Chemical Society·2026
関連記事をすべて見る

関連する実験動画

Updated: May 28, 2026

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

自由タンパク質の構造ダイナミクスは, difrraction で

Milo M Lin1, Dmitry Shorokhov, Ahmed H Zewail

  • 1Physical Biology Center for Ultrafast Science and Technology, Arthur Amos Noyes Laboratory for Chemical Physics, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|October 7, 2011
PubMed
まとめ
この要約は機械生成です。

タンパク質の展開のダイナミクスは,分子ダイナミクスのシミュレーションを使用して研究されました. アルファヘリクスの崩壊とシモシンベータにおけるコンフォーマーションアニリングは,温度ジャンプの大きさに依存する.

さらに関連する動画

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

関連する実験動画

Last Updated: May 28, 2026

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
08:03

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Published on: April 13, 2022

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
07:19

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Microcrystallography of Protein Crystals and In Cellulo Diffraction
09:35

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

科学分野:

  • バイオフィジックス 生物物理学
  • 構造生物学 構造生物学とは
  • コンピューティング・ケミストリー

背景:

  • アルファヘリクスは,生物学的マクロ分子における重要な構造モチーフである.
  • タンパク質の折りたたみ/展開を理解することは,分子生物学にとって極めて重要です.

研究 の 目的:

  • ヘリックス・コイル・トランジションのダイナミクスを解明し,チモシンベータ9 (Tβ9) で展開する.
  • 暫定的なマクロ分子構造の原子スケールの時空解像度を提供する.

主な方法:

  • 大規模に分布したアンサンブル収束分子動力学 (MD) シミュレーション.
  • ラディアル分布機能を利用した超高速電子 difraktion (UED) シミュレーション.
  • 分析は,ネイティブのアルファヘリカル型水素結合コンタクトメトリックを用いて行われました.

主要な成果:

  • アルファヘリル形状のモチーフの腐敗とTβ(9) の形状性解熱が観察されました.
  • これらのプロセスは,温度ジャンプの大きさに基づいて連続的にまたは競争的に発生します.
  • UEDとMDのシミュレーションは,基本的なコンフォメーションの相互変換のステップについての洞察を提供しました.

結論:

  • この研究は,Tβの展開のダイナミクス (解き放つ) の包括的なイメージを明らかにしています.
  • 温度ジャンプの大きさは,ヘリックス崩壊とコンフォーメーションアンニングの相互作用を左右します.