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関連する概念動画

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

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Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker...
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Kinetic Measurement and Real Time Visualization of Somatic Reprogramming08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

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The protocol presented in this study describes methods for the real-time monitoring of reprogramming progression via the kinetic measurement of positive and negative pluripotent stem cell markers using flow cytometry analysis. The protocol also includes the imaging-based assessment of morphology, and marker or reporter expression during iPSC...
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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

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Here, we present our established method to reprogram human somatic cells into transgene-free human iPSCs with Sendai virus, which shows consistent outcome and enhanced...
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In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors12:12

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This study demonstrates the reprogramming of somatic cells towards pluripotency in vivo without the generation of teratomas. We used hydrodynamic tail vein injection of plasmid DNA encoding the Yamanka factors to induce the in vivo reprogramming of adult hepatocytes into cells of enhanced...
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We demonstrate the protocol for the generation of induced pluripotent stem cells from human somatic cells using lentivirus-mediated delivery of the human factors Oct4, Sox2, Nanog, and Lin28. Pluripotency was confirmed by morphology and the presence of embryonic stem (ES) cell-specific...
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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関連する実験動画

Updated: Jan 20, 2026

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
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人間の卵子は,体細胞を再プログラムして,多能性の状態にします.

Scott Noggle1, Ho-Lim Fung, Athurva Gore

  • 1The New York Stem Cell Foundation Laboratory, New York, New York, USA.

Nature
|October 8, 2011
PubMed
まとめ

疾患モデリングのためのヒト卵細胞のゲノム交換は,発達停止のため失敗しました. しかし,体性ゲノムを無傷の卵細胞に追加することで,芽細胞の発達と多能幹細胞の派生が可能になった.

さらに関連する動画

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
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In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
12:12

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関連する実験動画

Last Updated: Jan 20, 2026

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Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

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Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

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In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors
12:12

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科学分野:

  • 生殖生物学 生殖生物学
  • 幹細胞科学 幹細胞科学とは
  • ヒューマン・ジェネティクス ヒューマン・ジェネティックス

背景:

  • 細胞置換療法 (Cell replacement therapy) は,人の退行性疾患の治療に有望である.
  • 患者特有の細胞を生成するには,体細胞を多能幹細胞に再プログラムする必要があります.

研究 の 目的:

  • ヒト卵細胞ゲノム交換を通じて,患者特有の多能幹細胞を生成する可能性を調査する.
  • 成功した再プログラミングと開発を制限する要因を特定する.

主な方法:

  • 人間の卵細胞は,体細胞核とゲノム交換を行いました.
  • あるいは,体細胞の核を無傷の卵細胞に加え,トリプロイド細胞を作り出した.
  • 派生細胞は,発達の可能性と多能性マーカーについて評価された.

主要な成果:

  • ゲノム交換は,トランスクリプションの異常で,後期的な解剖段階での発達停止につながった.
  • 健全な卵細胞に体ゲノムを加えた結果,芽細胞が発達した.
  • これらのブラストキストから派生した幹細胞系は,多能性を発揮し,3つの生殖層すべてに差異化しました.

結論:

  • 卵細胞のゲノムの除去は,ゲノム交換された卵細胞における発達障害の主な原因です.
  • 卵細胞を用いたヒト体細胞の再プログラミングは,卵細胞のゲノムが保持されている場合に実現可能である.
  • このアプローチは,疾患モデリングと潜在的な治療法のための患者固有の多能幹細胞の生成を可能にします.