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関連する概念動画

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...

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Updated: May 28, 2026

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

ATM コントロール メオティック・ダブル・ストランド・ブレイク・フォーメーション

Julian Lange1, Jing Pan, Francesca Cole

  • 1Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.

Nature
|October 18, 2011
PubMed
まとめ

ATMキナーゼは,SPO11の活性を抑制することによって,二分化過程中の二重鎖断裂 (DSB) の形成を抑制します. このネガティブなフィードバックループは,過度のDSBを防止し,メオティックエラーや性腺変異の予防に不可欠です.

科学分野:

  • 遺伝学 遺伝学とは
  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学

背景:

  • MEIOTIC再結合は,SPO11.11によって形成されたプログラムされた二重鎖断裂 (DSB) によって開始されます.
  • 適切なDSB形成は,同種の染色体ペアリングと分離に不可欠です.
  • 過剰なまたは不適切に修復されたDSBは,メオティック停止または変異につながる可能性があります.

研究 の 目的:

  • ミエオティック・ダブル・ストランド・ブレイク (DSB) の数を制御するメカニズムを調査する.
  • 分離過程中のDSB形成を調節するATMキナーゼの役割を決定する.
  • ATM欠乏症の個体における性腺不発症の分子基礎を解明する.

主な方法:

  • 野生型およびATM欠乏性マウス精子細胞におけるSPO11-オリゴヌクレオチド複合体の分析.
  • ATM変異の文脈におけるSPO11タンパク質レベルの遺伝子操作.
  • DNA損傷に対するATMキナーゼの活性化に関する研究.

主要な成果:

  • ATMが欠けている精子細胞は,SPO11-オリゴヌクレオチド複合体の10倍の増加を示し,DSBの形成が増加していることを示しています.
  • ATM欠乏症は,SPO11-オリゴヌクレオチドレベルがSPO11タンパク質レベルの変化に敏感になる.

さらに関連する動画

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

関連する実験動画

Last Updated: May 28, 2026

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

  • DSBによるATMキナーゼ活性化は,さらなるDSB形成を抑制するようです.

    • 結論:

    • ATMは,フィードバックループを通して,中性DSB形成の負の調節剤として作用します.
    • このATMによるDSBの制御は,過剰なDNA破裂を防止し,適切な半導体を確保するために不可欠です.
    • この発見は,アタキア・テランジエクタシアの患者における性腺変異の分子説明を提供する.