酵母熱ショック転写因子には,転写活性化ドメインが含まれており,その活動は非ショック条件下で抑制されます
J Nieto-Sotelo1, G Wiederrecht, A Okuda
1Division of Chemistry, California Institute of Technology, Pasadena 91125.
Cell
|August 24, 1990
まとめ
イーストの熱ショック転写因子 (HSTF) は,遺伝子転写の活性化と抑制のためのドメインを持っています. 温度調節ドメインは,熱ストレス中に酵母菌の生存に不可欠であり,脱圧メカニズムを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞のストレス反応は,
背景:
- 熱ショック遺伝子はストレスによって活性化されます.
- 酵母熱ショック転写因子 (HSTF) は,不活性から活性形態に切り替わると考えられています.
- 温度調節トランスクリプションにおけるHSTFの役割を理解することが鍵となる.
研究 の 目的:
- HSTFにおけるトランスクリプションの活性化と抑制を担当するドメインを特定する.
- HSTFにおける温度調節型アクティベーションドメインの存在と機能を調査する.
- 温度変化に反応して,HSTFが転写をどのように調節するかのモデルを提案する.
主な方法:
- 熱ショック転写因子 (HSTF) ドメインの分析.
- トランスクリプションの活性化と抑制の実験的調査.
- 熱ショック条件下における酵母細胞生存における特定のHSTFドメインの役割を評価する.
主要な成果:
- トランスクリプションの活性化に関与するHSTF内の特定のドメインを特定しました.
- 通常の温度で転写活性化を抑制するドメインを発見.
- HSTF.における温度調節された転写活性化ドメインの証拠を提供した.
- この領域が熱ショック温度下での酵母菌の生存に不可欠であることを実証した.
結論:
- HSTFは,トランスクリプションの活性化と抑制のための異なるドメインを有しています.
- HSTFの温度調節された活性化ドメインは,熱ストレス中の細胞生存に不可欠です.
- HSTFに対して,脱圧による温度調節による転写活性化のモデルが提案されています.
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