関連する実験動画
Updated: Jun 6, 2026

10:55
Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
Published on: November 5, 2012
cdc2のようなタンパク質は,Xenopusの卵エキスのDNA複製の開始に関与しています
Cell
|September 7, 1990
まとめ
クセノプスの卵抽出物はDNA複製を開始する. タンパク質p34cdc2を枯渇させると,開始は停止するが,延伸は停止しないので,真核生物のDNA複製を開始する上で重要な役割を果たしていることが示されている.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学 分子生物学
- バイオケミストリー バイオケミストリー
背景:
- 染色体DNAの複製は,細胞分裂における基本的なプロセスです.
- 細胞循環は,DNA複製の正確なタイミングを制御しています.
- クセノプスの卵エキスは,DNA複製を研究するために細胞のないシステムを提供します.
研究 の 目的:
- 染色体DNA複製を開始するXenopus p34cdc2の役割を調査する.
- p34cdc2がDNA複製の開始に不可欠かどうかを in vitroで判断する.
主な方法:
- 実験室内DNA複製試験に利用されたXenopus卵のエキス.
- アフィニティ減少 (p13suc1) または免疫減少を用いた抽出物から欠乏したp34cdc2.
- 劣化および再構成された抽出物におけるDNA複製の開始と延長を評価した.
主要な成果:
- p34cdc2-枯渇した抽出物はDNA複製を開始できませんでしたが,延長をサポートしました.
- 核の組み立ては,通常,枯渇した抽出物で発生しました.
- p34cdc2の再加法により,DNA複製の開始が回復しました.
結論:
- Xenopus p34cdc2は染色体DNA複製の開始に不可欠である.
- p34cdc2は,核の組み立てから独立して,開始段階で機能します.
- これらの発見は,真核細胞の細胞周期調節におけるp34cdc2の保存された役割を強調しています.
関連する概念動画
Replication in Eukaryotes
Overview
The Replisome
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
Restarting Stalled Replication Forks
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Replication in Eukaryotes
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.

