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関連する概念動画

Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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関連する実験動画

Updated: May 27, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

ncRNAとPc2のメチル化に依存する,核構造間の遺伝子移動は,遺伝子活性化プログラムを媒介する.

Liuqing Yang1, Chunru Lin, Wen Liu

  • 1Howard Hughes Medical Institute, University of California, San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093-0648, USA.

Cell
|November 15, 2011
PubMed
まとめ

ポリコンブ2タンパク質 (Pc2) のメチル化は,ノンコーディングRNA (ncRNA) を通して,核構造間の成長遺伝子移動を制御する. これは,核アーキテクチャと遺伝子発現の調節を結びつける.

さらに関連する動画

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

関連する実験動画

Last Updated: May 27, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
09:42

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • エピジェネティクス エピジェネティクス

背景:

  • ユカリオット核は,ノンコーディングRNA (ncRNA) にリンクされた構造を持っています.
  • 転写を調節するこれらの構造の役割はよく理解されていません.

研究 の 目的:

  • 核建築構造,ncRNAs,および転写規則の間の関係を調査する.
  • 成長制御遺伝子が核内に移転するメカニズムを解明する.

主な方法:

  • ポリコンブ2タンパク質 (Pc2) のメチル化と脱メチル化の役割を調査した.
  • Pc2のTUG1およびMALAT1/NEAT2.2のncRNAへの結合を研究した.
  • ポリコンブ体 (PcG) とインタークロマチン粒 (ICG) の間の遺伝子転移への影響を調査した.
  • コアプレッサー/コアクティベーター・アセンブリとヒストン・コード・リーダーの効果を分析した.
  • E2F1 SUMOylationと遺伝子活性化におけるNEAT2-Pc2相互作用の役割を評価した.

主要な成果:

  • Pc2メチル化状態は,PcGとICGの間の成長制御遺伝子の移転を決定する.
  • Pc2はメチル化状態に基づいて特定のncRNA (PcGではTUG1,ICGではMALAT1/NEAT2) と結合する.
  • ncRNAは,調節性タンパク質複合体の組み立てを促進し,ヒストンのマーク認識に影響を与えます.
  • NEAT2が非メチル化Pc2に結合すると,E2F1SUMOylationが促進され,成長遺伝子が活性化されます.

結論:

  • 分子経路は,亜核構造特異のncRNAとタンパク質メチレーションを遺伝子移転に結びつける.
  • この経路は,核アーキテクチャと転写制御を結びつけることで,調整された遺伝子発現プログラムを達成します.
  • この発見は,核組織におけるダイナミックな変化を通して,成長制御遺伝子を調節する新しいメカニズムを明らかにしている.