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関連する概念動画

M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

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関連する実験動画

Updated: May 27, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

ミトスの脱出時にCdk基板のオーダーされたデフォスフォリレーションのための定量モデル.

Céline Bouchoux1, Frank Uhlmann

  • 1Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, London WC2A 3LY, UK.

Cell
|November 15, 2011
PubMed
まとめ
この要約は機械生成です。

ミトスの脱出は,秩序付けられた出来事を伴う. 芽生えた酵母では,Cdk基板の固有の脱酸化タイミングが,フォスファターゼとキナーゼの活動が細胞サイクル進行をどのように制御しているかを明らかにします.

さらに関連する動画

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

関連する実験動画

Last Updated: May 27, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • ミトスの脱出は姉妹染色体分離に続いており,サイクリン依存キナーゼ (Cdk) によって多数の基質の脱リン酸化を伴う.
  • ミトスの脱出過程におけるこれらの脱酸化現象の時間順序を規定する正確なメカニズムは,まだ十分に理解されていません.

研究 の 目的:

  • 芽生える酵母におけるミトシス脱出過程におけるCdk基板脱酸化のオーダーされたタイミングを調査する.
  • ミトスの出口の間に起こる出来事の時間的な順序を確立するメカニズムを解明する.

主な方法:

  • サイクリン依存キナーゼ (Cdk) とCdc14フォスファターゼの活性を調節するインビボ実験.
  • Cdk基板のリン酸化と脱リン酸化のインビトロ運動分析.
  • ミトスの脱出イベントの時間順序に関する異なるモデルをテストする.

主要な成果:

  • 連続したミトスの脱出イベントに関与するCdk基板の脱酸化は,秩序付けられたタイミングで発生します.
  • リン酸塩とキナーゼの比率は,ミトスの脱出の間に徐々に変化します.
  • Cdk基板は,この比率の変化の明確な値に反応し,オーダーされた脱リン化につながります.

結論:

  • フォスファタゼとキナーゼの比率の漸進的なシフトは,ミトーシス離脱イベントの順序を定めるための定量的メカニズムを提供します.
  • この研究は,細胞サイクル進行の定量的モデルに対するメカニズム的説明を提供します.
  • 発芽酵母における発見は,基本的な細胞循環調節に関する洞察を提供します.