腫瘍死滅因子 (TNF) の非分泌性細胞表面変異体は,細胞間接触によって殺す
1Department of Molecular Biology, Cetus Corporation, Emeryville, California 94608.
Cell
|October 19, 1990
まとめ
腫瘍死滅因子 (TNF) は,分泌性およびトランスメブラン性という2つの形態があります. TNFのトランスメブラン形態は,分泌形態とは異なり,細胞との接触を通じて標的細胞を直接殺します.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- 腫瘍死滅因子 (TNF) は,腫瘍の回帰とセプティックショックやリウマチ性関節炎などの様々な病理に関与しています.
- TNFは主に2つの形態で存在します: 17kDの分泌成分と26kDのトランスメブラン形式です.
- これらのTNF形態の独特の生物学的活動は完全に理解されていません.
研究 の 目的:
- TNFの26kdトランスメブラン形態の生物学的活性を調べる.
- 細胞内TNFと分泌TNFの細胞機能を比較する.
- TNFが細胞毒性効果を発揮するために内部化が必要かどうかを判断する.
主な方法:
- TNF.の食べられない,そして分泌されるだけの突然変異体の生成.
- これらのエンジニアリングされたTNF変異体の生物学的活動を研究する.
- 腫瘍およびウイルスに感染した細胞に対するTNFの細胞毒性効果の評価.
主要な成果:
- 26kdトランスメブランのTNFの食べられない変異体は,直接の細胞対細胞接触を通じて標的細胞を効果的に殺します.
- TNFは,細胞死を誘発するために,標的細胞によって内化する必要はありません.
- TNFのトランスメブラン形態は局所的な細胞毒性を媒介する.
結論:
- TNFの26kDの統合型トランスメブラン型は,分泌成分と比較して独特の生物学的活性を持っています.
- トランスメブランTNFは,腫瘍細胞やその他の標的を局所的に殺すためにin vivoで機能する可能性が高い.
- この局所的な作用は,TNFの分泌形態で観察された全身の生物活性と対照的です.
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