ゴルギからプラズマ膜への大量フローの速度
A Karrenbauer1, D Jeckel, W Just
1Institut für Biochemie I, Universität Heidelberg, Federal Republic of Germany.
Cell
|October 19, 1990
まとめ
研究者たちは,生体細胞が分泌する水溶性スフィンゴミエリンアナログを合成した. この研究では,このアナログがゴルギ装置を通して輸送される様子を追跡し,細胞の脂質処理と輸送経路に関する洞察を明らかにしました.
科学分野:
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
- 脂質代謝についてです.
背景:
- スフィンゴミエリンとグリコリピドは,必須細胞脂質である.
- その合成と輸送には,ゴルギ装置が関与しています.
- これらのプロセスを理解することは,細胞の機能の鍵です.
研究 の 目的:
- スフィンゴミエリンの水溶性アナログを合成する.
- 生体細胞におけるゴルギー装置によるその利用と輸送を調査する.
- その分泌の動態を決定し,ER-to-Golgi輸送率を推論する.
主な方法:
- 切断されたC8C8セラミドアナログの合成.
- 中国ハムスター卵巣 (CHO) 細胞でインキュベーション.
- 37°Cと30°Cでのアナログ分泌率の測定
- ERのルメナルマーカーの分泌率と比較.
主要な成果:
- 切断されたセラミドアナログは水溶性だが,細胞に浸透性がある.
- 生きている細胞は,そのアナログを使って,水溶性断片化フォスフォリピドとグリコリピドを産生し分泌した.
- 37°Cで,アナログは約10分の半減期で分泌されました.
- 30°Cでは,アナログの分泌半減期は~14分であり,迅速なERからゴルギ輸送を示唆しています.
結論:
- 切断されたスフィンゴミエリンアナログは,細胞機械によって処理され,分泌することができます.
- ゴルギ装置は,このアナログを利用して,切断された脂質を生成することができます.
- ERからGolgiへの輸送は高速で,ERの半分が排出されるまでの半減時間は約4分です.
関連する概念動画
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
01:44Introduction to Membrane Traffic
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:26Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:44Introduction to Membrane Traffic
The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...