関連する実験動画
Updated: Jun 17, 2026

14:29
Examination of Thymic Positive and Negative Selection by Flow Cytometry
Published on: October 8, 2012
TCRガンマ・デルタ+T細胞のエクストラティミック・セレクションは,IIクラスメジャー・ヒストコンパティビリティ・コンプレックス分子による
L Lefrancois1, R LeCorre, J Mayo
1Department of Cell Biology, Upjohn Company, Kalamazoo, Michigan 49001.
Cell
|October 19, 1990
まとめ
メジャー・ヒストコンパティビリティ・コンプレックス (MHC) 抗原は,CD8+内皮細胞リンパ球 (IEL) のT細胞受容体 (TCR) ガンマデルタ使用に影響を与えます. これは,これらの細胞のエクストラティミックな選択メカニズムを示唆しています.
科学分野:
- 免疫学 免疫学とは
- T細胞の生物学について
- 胃腸免疫学 胃腸免疫学とは
背景:
- CD8+内皮細胞リンパ球 (IELs) は,腸内膜の重要な免疫細胞である.
- T細胞受容体 (TCR) ガンマデルタの使用は多様で,その調節は完全に理解されていません.
- IELレパートリーを形成するMHCアンチゲンの役割は,活発な調査分野です.
研究 の 目的:
- CD8+ IELにおけるTCRガンマデルタ利用に対するMHC抗原の影響を調査する.
- MHCが特定のTCR Vデルタ地域の選択に影響を与えるかどうかを判断する.
- CD8+ IELにおけるTCRガンマデルタ選択のメカニズムを探求する.
主な方法:
- TCRのガンマデルタのパン反応性およびVデルタ4領域に特異的なモノクローナル抗体 (MAbs) を利用した.
- 様々なMHCハプロタイプを持つマウスのIELを分析し,F1および再結合性近親種株を含む.
- 胸腺なしのキメリックマウスで検査されたTCRガンマデルタ選択.
主要な成果:
- Vデルタ4+CD8+IELの有意に高い割合は,H-2k MHCハプロタイプを持つマウスで観察されました.
- F1および再結合株の分析は,Vデルタ4の使用量の増加にはMHCクラスII,特にI-E発現が必要であることを示しました.
- TCRガンマデルタ選択は,キメリックマウスの胸腺とは独立して発生した.
結論:
- MHC抗原は,CD8+ IELにおけるTCRガンマデルタのエクストラティミック選択において重要な役割を果たします.
- この発見は,ガンマデルタTCRsのための新しいエクストラティミック選択的メカニズムを示唆しています.
- CD8+ IELは,MHCクラスIIに制限された抗原認識能力を有することがあります.
関連する概念動画
Antigens Involved in Adaptive Immunity
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Antigen Processing Pathways
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

