HIV-1 gp120 V1/V2ドメインの構造は,広範囲中和抗体PG9を持つ
Jason S McLellan1, Marie Pancera, Chris Carrico
1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nature
|November 25, 2011
まとめ
ヒト免疫不全ウイルス-1 (HIV-1) 封筒V1/V2領域は,免疫逃避の鍵です. 研究者は,PG9抗体と結合するV1/V2の構造を決定し,新しい中和メカニズムを明らかにした.
科学分野:
- 免疫学 免疫学とは
- ウイルス学 ウイルス学 ウイルス学
- 構造生物学 構造生物学とは
背景:
- HIV-1 gp120エンベロープグリコタンパク質の変数領域1と2 (V1/V2) は,ウイルスの免疫回避に不可欠です.
- これらの領域は,高い配列多様性およびN-結合グリコシル化によって保護されており,抗体中和に挑戦しています.
研究 の 目的:
- HIV-1 V1/V2領域の構造を,中和抗体PG9.9との複合体として決定する.
- PG9のような抗体がV1/V2領域経由でHIV-1を認識し,中和するメカニズムを解明する.
主な方法:
- X線結晶学を用いてV1/V2-PG9複合体の構造を決定した.
- 構造分析はV1/V2,グリカン,抗体間の相互作用を特徴付けるために使用されました.
主要な成果:
- V1/V2領域は4鎖のβシートドメインを形成し,多様性とグルコース化がループに局所化している.
- PG9の認識には,静電性,配列独立性,およびグリカン相互作用が含まれており,グリカンは結合表面に大きく貢献しています.
- CH04とPGT145の抗体は,アニオンループを含む共通のグリカン浸透機構を共有しています.
結論:
- この研究では,HIV-1 V1/V2領域とその中和抗体との相互作用を構造的に定義しています.
- 高度グリコシル化抗原の抗体認識のためのパラダイムが特定され,特定のグリカンとβシート鎖を含むV1/V2領域の脆弱性を強調しています.
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Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:25Antibody Structure and Classes
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.