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関連する概念動画

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

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関連する実験動画

Updated: May 26, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

コファクター結合は,ホックスタンパク質間のDNA結合特異性の潜在的差異を誘発する.

Matthew Slattery1, Todd Riley, Peng Liu

  • 1Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168(th) Street, HHSC 1104, New York, NY 10032, USA.

Cell
|December 14, 2011
PubMed
まとめ
この要約は機械生成です。

転写因子は,コファクターとの複合体を形成する際に,新しいDNA結合能力を獲得する. この研究は,ドロソフィラホックスタンパク質が,Extradenticle-Homothoraxコファクターで認識特性をどのように変化させるかを明らかにしています.

さらに関連する動画

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

関連する実験動画

Last Updated: May 26, 2026

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • 転写因子ファミリーは,DNA結合の類似性を共有していますが, in vivo では異なる機能を持っています.
  • 転写因子はしばしばDNAをマルチタンパク質複合体として結合し,DNA結合特異性の変化を示唆する.

研究 の 目的:

  • 転写因子複合体の形成がDNA結合特異性を修正するかどうかを調査する.
  • 転写因子複合体のDNA配列親和性を決定する方法を開発する.

主な方法:

  • 実験・計算プラットフォームであるSELEX-seqを開発しました.
  • SELEX-seqをDrosophila Hoxの8種類のタンパク質とExtradenticle-Homothorax (Exd) のコファクターすべてに適用しました.

主要な成果:

  • ドロソフィラ・ホックスタンパク質は,Exd.と複合すると,新しいDNA認識特性を示す.
  • Exd-Hoxの特異性は3つのクラスに分類され,Hox遺伝子のコリネアリティと一致しています.
  • DNA構造の予測は,前後ホックスタンパク質に対する異なるマイナー・グリューブ・トポグラフィの偏好を示している.

結論:

  • Exd と Hox タンパク質のようなコファクター相互作用は,新興の DNA 認識特性を生み出します.
  • これらの新興特性は,転写因子のインビボ特異性に貢献します.