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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Published on: August 13, 2016
STARD9/Kif16aキネシンはミトの微小管と結合し,スパインドルポール組立を調節する
Jorge Z Torres1, Matthew K Summers, David Peterson
1Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA. torres@chem.ucla.edu
Cell
|December 14, 2011
まとめ
研究者らは,ミトーシス・スパインドルの組み立てに不可欠なタンパク質であるSTARD9を特定した. 癌細胞におけるSTARD9の減少はミトス停止とアポトーシスを引き起こし,STARD9ががん治療の潜在的な標的であることを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子腫瘍学 分子腫瘍学
- 癌の治療薬について
背景:
- ミトス・スピンドルは,細胞分裂中の正確な染色体分離に不可欠です.
- 癌細胞はしばしば異常なミトーシス・スピンドルを示し,抗ミトーシス治療の標的となる.
- スピンドルの組成を調節する新しいタンパク質を特定することは,新しいがん治療の開発の鍵です.
研究 の 目的:
- ミトスのスパインドルの組成に不可欠なタンパク質を特定し,特徴づけること.
- 抗真菌薬の標的として機能するミトーシスの新しい調節体を発見すること.
- スピンドル形成におけるSTARD9の役割と,治療標的としてのその可能性を調査する.
主な方法:
- 592のミトス微小管共浄タンパク質 (MMCP) のプロテオミクおよび遺伝子解析.
- STARD9を含む特定されたタンパク質の機能的特徴付けは,細胞ベースの測定法を使用して行われます.
- スピンドル組立,周心地物質 (PCM) の安定性,アポトーシス誘導におけるSTARD9の役割の評価.
主要な成果:
- キネシン-3ファミリーのメンバーであるSTARD9は,スパインドルアセンブリの重要なレギュレータとして特定されました.
- STARD9はセンターソームに局所化し,周心地物質 (PCM) を安定させる.
- STARD9の枯渇は,PCMの断片化,多極スパインドルの形成,スパインドルアセンブリチェックポイント (SAC) の活性化,ミトスの停止,およびアポトーシスにつながる.
- STARD9の枯渇はタクソールと連携し,ミトーシス細胞死を増強する.
結論:
- STARD9は,適切なスパインドル組立と染色体分離に不可欠な重要なミトーシスキネシンです.
- STARD9は,PCMの完全性を維持し,異常なスパインドル形成を防止する上で重要な役割を果たします.
- STARD9は,がん治療,特に併用治療において,有望な新しい抗菌体標的を代表しています.
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