Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Membrane Composition Influences Expression Yield of Plant Cytochrome P450s in <i>E. coli</i> Lysate-Based Cell-Free Systems.

ACS synthetic biology·2026
Same author

A standardized workflow for kinetic metabolic model curation and dissemination.

PLoS computational biology·2026
Same author

Evaluating the limitations of Bayesian metabolic control analysis.

PLoS computational biology·2026
Same author

Cell-Free-Based Thermophilic Biocatalyst for the Synthesis of Amino Acids from One-Carbon Feedstocks.

ACS synthetic biology·2025
Same author

Systematic Mapping of Bacterial CRISPRa Systems for Synergistic Gene Activation Reveals Antagonistic Effects.

ACS synthetic biology·2025
Same author

Hydrogel-Immobilized Multienzyme Systems for Cell-Free Chemical Bioproduction.

ACS synthetic biology·2025

関連する実験動画

Updated: May 26, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

遺伝子発現を定量的にプログラムするためのRNA装置のモデル駆動工学.

James M Carothers1, Jonathan A Goler, Darmawi Juminaga

  • 1California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley CA 94720, USA.

Science (New York, N.Y.)
|December 24, 2011
PubMed
まとめ
この要約は機械生成です。

科学者たちは,遺伝子発現制御を設計するために,機械的モデリングとRNA折り畳みシミュレーションを使用して,合成生物学のための新しい設計アプローチを開発しました. この方法により,様々な用途のためのRNAベースの遺伝子装置の予測可能で正確なエンジニアリングが可能になります.

さらに関連する動画

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

関連する実験動画

Last Updated: May 26, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

AQRNA-seq for Quantifying Small RNAs
05:12

AQRNA-seq for Quantifying Small RNAs

Published on: February 2, 2024

科学分野:

  • 合成生物学 合成生物学とは
  • 分子生物学は分子生物学である.
  • バイオケミカルエンジニアリング

背景:

  • 複雑な合成生物学的装置を設計するためのモデルとシミュレーションツールの利用可能性は限られている.
  • 予測可能な遺伝子発現制御を設計するための堅牢な方法が必要である.

研究 の 目的:

  • RNA調節遺伝子装置の設計のための設計主導的なアプローチを策定する.
  • RNAベースのコンポーネントを使用して遺伝子発現の定量的に予測可能な制御を可能にします.

主な方法:

  • 機械的モデリングと運動RNAの折り畳みシミュレーションを使用しました.
  • リボエンザイム,メタボライト制御,アパエンザイム調節式発現装置を組み立て,特徴づけました.
  • 設計戦略をin vitro,in vivo,in silico分析で検証しました.

主要な成果:

  • 28種類のEscherichia coli発現装置を成功裏に設計しました.
  • 予測された遺伝子発現レベルと測定された遺伝子発現レベル (r = 0.94) の間の優れた定量的な一致を達成しました.
  • 代謝経路におけるRNA制御を設計するための技術の実証された応用.

結論:

  • 開発された設計アプローチは,RNAの機能を研究するための枠組みを提供します.
  • 生物化学的および生物物理的モデリングの潜在力を強調し,生物学的設計方法の進歩を図る.
  • 機能的に複雑で予測可能に制御された合成生物システムの作成を可能にします.