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リボソームは新生タンパク質の折り畳みを調節する
Christian M Kaiser1, Daniel H Goldman, John D Chodera
1Institute for Quantitative Biosciences , University of California-Berkeley, CA 94720, USA.
Science (New York, N.Y.)
|December 24, 2011
まとめ
リボソームはタンパク質の折りたたみに影響を与えます. それはネイティブ状態の達成を遅らせますが,新生ポリペプチドの誤折りや集積を防止し,効率的なデノボ折りを促進します.
科学分野:
- 分子生物学は分子生物学である.
- バイオフィジックス 生物物理学
背景:
- タンパク質は,リボソームによって合成された後に活性化するために適切な折りたたみを必要とします.
- タンパク質の折りたたみにおけるリボソームの役割は仮説化されているが,実験的に証明するのは困難である.
研究 の 目的:
- 新生ポリペプチドの折りたたみに対するリボソームの影響を調査する.
- リボソームがタンパク質の折り畳みの運動学と忠誠性に影響するかどうかを判断する.
主な方法:
- 光学ピンチを使った実験システムの開発.
- 単一リボソームに結合した,in vitroトランスレーション中に停滞した新生ポリペプチドの研究.
- T4ライソ酵素をモデルタンパク質として利用する.
主要な成果:
- リボソームは,自由タンパク質と比較して,T4リゾーシムの安定した三次相互作用の形成とネイティブ状態の達成を遅らせます.
- 溶液中の不完全なポリペプチドは,折り合いを誤り,集積する傾向があり,リボソームの近くでは折り合いに適しています.
- リボソームの表面環境は,折り畳み能力をサポートしているようです.
結論:
- リボソームは,合成を超えて重要な役割を果たし,効率的なデノボタンパク質の折り畳みを積極的に促進します.
- リボソームに結合した新生鎖は,自由ポリペプチドと比較して,明確な折り畳み行動を示す.
- リボソームは,ネイティブ状態の達成を促進し,その表面の近くで誤った折り畳みを防ぐ.
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