関連する概念動画
02:31Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
02:31Nuclear Export of mRNA
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
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関連する実験動画
Updated: May 11, 2026
10:56
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Published on: May 17, 2014
プロモーター要素は,シトプラズマのmRNAの分解を調節する.
Almog Bregman1, Moran Avraham-Kelbert, Oren Barkai
1Department of Molecular Microbiology, Technion-Israel Institute of Technology, Haifa 31096, Israel.
Cell
|December 27, 2011
まとめ
イーストのプロモーター,特にアップストリーム活性化配列 (UAS) とRap1pタンパク質は,転写後のメッセンジャーRNA (mRNA) 崩壊率を制御することができます. この発見は,転写後の遺伝子発現を調節するプロモーターの新たな役割を明らかにしています.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- 遺伝子規制 遺伝子規制
背景:
- プロモーターは,転写を開始するために重要なDNA配列であり,トランス作用因子によって調節されます.
- プロモーターに関する従来の理解は,遺伝子合成におけるプロモーターの役割に限られており,転写後の調節ではない.
研究 の 目的:
- 酵母プロモーターが転写後のmRNA分解率に影響を与えるかどうかを調査する.
- mRNAの安定性を調節する特定のプロモーター要素と要因を特定する.
主な方法:
- 酵母におけるレポーター遺伝子システムを活用して,mRNAの分解運動を評価した.
- レポーター遺伝子のアップストリーム活性化配列 (UAS) を操作した.
- mRNAの分解におけるRap1p結合部位とRap1pタンパク質の役割を調査した.
主要な成果:
- レポーター遺伝子のUAS配列を変化させることで,トランスクリプトが変化することなく,その衰退率が変化します.
- Rap1pと2つのRap1p結合部位を持つ特定のcis要素は,mRNAの崩壊を促進するのに十分でした.
- Rap1pは,特定の内生mRNAの合成と分解の両方を刺激することが判明しました.
結論:
- イーストプロモーターは,UASのような要素とRap1pのようなタンパク質を通じて,細胞質のmRNAの分解を調節することができます.
- プロモーター結合因子は,メッセンジャーリボヌクレオプロテイン (mRNP) の組成に影響を与え,mRNAの安定性に影響を与える可能性があります.
- プロモーターは,mRNAの合成と分解の調整において二重の役割を果たし,全体的なmRNAレベルに影響を与えます.