Pr光イソメリゼーション中のフィトクロームの方向変化のリアルタイム追跡
Yang Yang1, Martin Linke, Theodore von Haimberger
1Department of Physics, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany.
Journal of the American Chemical Society
|January 11, 2012
まとめ
光によって誘発されるフィトクロームのZ-to-Eフォトイソメリゼーションは,刺激状態のリングDを30ps回転させる. 光受容体シグナル伝達に不可欠なこのプロセスは,単一の回転とタンパク質の相互作用によって駆動されます.
科学分野:
- バイオフィジックス 生物物理学
- フォトケミストリー フォトケミストリー
- 分子生物学は分子生物学である.
背景:
- タンパク質に結合した染色体の光異性化が,光受容体の機能の基礎となっている.
- フィトクロームは,様々な生物の重要な光感受性タンパク質です.
研究 の 目的:
- Pr Cph1Δ2 フィトクロームのZ-to-Eフォトアイソメリゼーションメカニズムを調査する.
- フォトイソメリゼーションプロセスの構造動力学と時間定数を決定する.
主な方法:
- ポラライゼーション解像度フェムト秒可視ポンプ赤外線探査スペクトロスコーピーを利用しました.
- 光反応の動力学と,興奮状態と基底状態における構造的変化を分析した.
主要な成果:
- 2つの光反応時間定数を特定しました: 4.7 ± 1.4 psと 30 ± 5 ps.
- 興奮状態のリングDのリアルタイム回転を30 ± 5 psの時間常数で観測した.
- 完成したリングDの回転は,電子的に興奮した状態で起こります.
結論:
- フォトアイソメリゼーションは,Pr*状態のC(15) C(16) メチンブリッジの周りを1回回転することで開始されます.
- このプロセスは,周囲のタンパク質環境との拡散相互作用を伴う.
- これらの発見は,フィトクローム信号を制御する超高速構造的ダイナミクスを明らかにします.
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