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関連する概念動画

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

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関連する実験動画

Updated: May 10, 2026

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
08:55

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Published on: March 26, 2012

後期ポリオマウイルスRNAにおける複数の5'端末キャップ構造.

A J Flavell, A Cowie, S Legon

    Cell
    |February 1, 1979
    PubMed
    まとめ

    ポリオーマウイルスの遅いmRNAは,ユニークな5'端末キャップ構造を持っています. サイズが異なるこれらのキャプテッドリーダー配列は,スプライシングを通じてメインmRNAボディに結合されます.

    科学分野:

    • 分子生物学は分子生物学である.
    • ウイルス学 ウイルス学 ウイルス学
    • RNA バイオケミストリー

    背景:

    • マウス細胞におけるポリオマウイルス感染は,ウイルス特異のRNAを産生する.
    • ウイルスのRNAの構造を理解することは,遺伝子発現機構の解読に不可欠です.

    研究 の 目的:

    • ポリオマウイルスの特異的な後期RNAの5'端蓋構造を特定し,特徴づけること.
    • これらのキャップ構造のゲノム位置と存在を,異なる後期mRNA種で決定する.

    主な方法:

    • RNase T1,T2,A,P1の消化を用いた32Pラベル付きウイルスRNAの分析.
    • RNA構造を分断するための二次元ゲル電泳.
    • カップ構造をポリオマウイルスゲノムにマッピングする.

    主要な成果:

    • 7つの主要なキャップI構造 (m7GpppNm1pN2p) と4つのキャップII派生 (m7GpppNm1pNm2pN3p) がウイルスのRNAで特定されました.
    • これらのキャップ構造は,ウイルスゲノムの特定の領域 (66-71マップ単位) にマッピングされます.
    • すべての特定されたキャップ構造は,精製された16S,18S,19Sの遅いmRNAで発見されました.

    さらに関連する動画

    In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
    08:58

    In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

    Published on: May 1, 2019

    Chemical Triphosphorylation of Oligonucleotides
    13:19

    Chemical Triphosphorylation of Oligonucleotides

    Published on: June 2, 2022

    関連する実験動画

    Last Updated: May 10, 2026

    In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection
    08:55

    In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

    Published on: March 26, 2012

    In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
    08:58

    In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells

    Published on: May 1, 2019

    Chemical Triphosphorylation of Oligonucleotides
    13:19

    Chemical Triphosphorylation of Oligonucleotides

    Published on: June 2, 2022

    結論:

    • ポリオマウイルスの遅いmRNAは,多様なリーダー配列でカバーされています.
    • これらのキャップされたリーダーは,スプライシングメカニズムを通じてmRNAボディに結合します.
    • この発見は,ウイルスのmRNAの転写後の処理に関する洞察を提供します.