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関連する概念動画

Biosynthesis of Nucleic Acids01:28

Biosynthesis of Nucleic Acids

Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...

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Updated: May 25, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

プログラム可能なワンポット・マルチステップの有機合成で,DNAの接点を使用しています.

Mireya L McKee1, Phillip J Milnes, Jonathan Bath

  • 1Department of Physics, University of Oxford, Clarendon Laboratory, Parks Road, Oxford, OX1 3PU, UK.

Journal of the American Chemical Society
|January 27, 2012
PubMed
まとめ
この要約は機械生成です。

この研究は,制御されたオリゴマーの生産のための新しいDNAテンプレート合成システムを導入しています. この方法は,単一のポットで効率的な並列合成を可能にし,複雑な分子のための反応ステップを削減します.

さらに関連する動画

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

関連する実験動画

Last Updated: May 25, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 合成化学 合成化学とは

背景:

  • オリゴマーの多段階合成は複雑で,多くの場合,複数の反応ステップと浄化段階を必要とします.
  • 現在の方法は,オリゴーマー長さに合わせて変化する反応条件によって制限されることがあります.
  • パラレル合成と結合戦略は,総合的な合成時間を短縮することができますが,慎重に制御する必要があります.

研究 の 目的:

  • 多段階のDNAテンプレート合成のための新しいシステムを開発する.
  • 連続制御されたオリゴーマー合成を常時反応環境で可能にする.
  • 単一の反応容器内で並列合成と結合を容易にする.

主な方法:

  • 合成プロセスを制御するために,DNAの接点の連続的な形成を利用します.
  • 反応物質をDNAアダプターに結合し,DNAテンプレート鎖にハイブリッド化します.
  • 繰り返し合成のためのハイブリッド化と反応のステップを繰り返す.
  • 必要なすべての反応性モノマーを含む単一のポットで合成を行う.
  • パラレル合成からの結合産物で,全体的なステップを減らします.

主要な成果:

  • 制御された多段階のDNAテンプレート合成のためのシステムを実証しました.
  • 一定の反応環境で,配列制御されたオリゴーマー合成を達成した.
  • 同じ容器内の異なるオリゴマーの並列合成を可能にしました.
  • ステップを減らすために並列合成製品の結合を展示しました.

結論:

  • 開発されたDNAテンプレート型システムは,効率的なオリゴマー合成のための堅牢なプラットフォームを提供します.
  • この方法は,単一のポットでシーケンスの制御と並列処理を可能にします.
  • このアプローチは,複雑な合成を簡素化し,反応ステップを削減し,生産性を高めます.