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Hsp90のストレスは,アヌプロイディの誘導により,細胞の急速な適応を強化する
Guangbo Chen1, William D Bradford, Chris W Seidel
1Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, Missouri 64110, USA.
Nature
|January 31, 2012
まとめ
ストレスは,芽生えた酵母菌で不均一な染色体数であるアヌプロイディを誘発することができます. Hsp90と関連したこの染色体不安定は,迅速な適応と耐薬性を促進し,進化におけるHsp90の新しい役割を明らかにする.
科学分野:
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
- 進化生物学の進化生物学について
背景:
- アヌプロイディは,異常な染色体数であり,がんでは一般的であり,ストレス下での適応特性を授与します.
- 最近の研究では,アヌプロイド症は様々な状態における適応を誘発する大きな効果の突然変異であると強調しています.
研究 の 目的:
- プレイオトロピクストレスが芽生えた酵母 (Saccharomyces cerevisiae) のアヌプロイディを誘発できるかどうかを調査する.
- ストレス誘発の染色体不安定性と適応におけるHsp90チャペロン複合体の役割を調査する.
主な方法:
- Hsp90の阻害と芽生えた酵母における熱ショックによるストレス誘発.
- 染色体不安定性とカリオタイプ多様性を分析する.
- アヌプロイドコロニーの薬剤耐性の遺伝的基礎を調査する.
主要な成果:
- タンパク質毒性ストレス (Hsp90阻害,熱ショック) は,染色体不安定性とカリオタイプ多様性を著しく増加させた.
- 誘発された不安定性は,キネトコア組立におけるHsp90の役割と関連しています.
- Hsp90の阻害により,薬剤耐性コロニーは染色体XVの増加,複数の遺伝子を含む定量的な特徴をもたらしました.
- 短期間のHsp90のストレス被曝は,アヌプロイド症経由で他の細胞毒性化合物への適応を加速した.
結論:
- アヌプロイド症は,真核生物におけるストレス誘発性変異であり,急速な現象型進化と薬剤耐性を促進する.
- Hsp90は,ストレス下での適応特性の出現を調節する上で重要な役割を果たします.
- この研究では,Hsp90がストレス誘発性アヌプロイド症と適応を媒介する新しい機能を明らかにしています.
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